CAJ | 학술논문

目的:研究酵母多糖(zymosan)对S_(180)荷瘤小鼠抗氧化状态和免疫功能的影响.方法:将昆明小鼠70只随机分为7组,第1组作为正常组,另6组将S_(180)瘤细胞悬液接种于小鼠右前肢腋皮下制备荷瘤小鼠动物模型,分为zymosan低、中、高剂量组和环磷酰胺(Cy)组、肿瘤对照组及zymosan联合Cy治疗组,灌胃第7 d接种S_(180)瘤,第19 d后测定小鼠肝匀浆中丙二醛(MDA)含量、谷胱甘肽过氧化酶(GSH-Px)和超氧化物歧化酶(SOD)活力,评价zymosan对小鼠抗氧化能力的作用.采用逆转录-聚合酶链反应(RT-PCR)检测IL-2、TNF-α、TGF-β_1 mRNA的表达,评价zymosan对小鼠免疫功能的影响.结果:zymosan各剂量组均能提高肝脏中GSH-Px活性和SOD活力,但zymosan高剂量组显著降低MDA水平(P＜0.01).zymosan各剂量组荷瘤小鼠肿瘤组织内TNF-α、IL-2 mRNA相对表达量明显高于Cy组和肿瘤对照组(P＜0.01),同时下调TGF-β_1水平.zymosan高剂量对小鼠S_(180)瘤有明显抑制作用,且与Cy合用后有协同作用,并提高了小鼠的TNF-α、IL-2 mRNA的表达.结论:zymosan的抑瘤机制可能主要是抗氧化作用和免疫调节作用,且作用与剂量有关.
목적:연구효모다당(zymosan)대S_(180)하류소서항양화상태화면역공능적영향.방법:장곤명소서70지수궤분위7조,제1조작위정상조,령6조장S_(180)류세포현액접충우소서우전지액피하제비하류소서동물모형,분위zymosan저、중、고제량조화배린선알(Cy)조、종류대조조급zymosan연합Cy치료조,관위제7 d접충S_(180)류,제19 d후측정소서간균장중병이철(MDA)함량、곡광감태과양화매(GSH-Px)화초양화물기화매(SOD)활력,평개zymosan대소서항양화능력적작용.채용역전록-취합매련반응(RT-PCR)검측IL-2、TNF-α、TGF-β_1 mRNA적표체,평개zymosan대소서면역공능적영향.결과:zymosan각제량조균능제고간장중GSH-Px활성화SOD활력,단zymosan고제량조현저강저MDA수평(P＜0.01).zymosan각제량조하류소서종류조직내TNF-α、IL-2 mRNA상대표체량명현고우Cy조화종류대조조(P＜0.01),동시하조TGF-β_1수평.zymosan고제량대소서S_(180)류유명현억제작용,차여Cy합용후유협동작용,병제고료소서적TNF-α、IL-2 mRNA적표체.결론:zymosan적억류궤제가능주요시항양화작용화면역조절작용,차작용여제량유관.
To study the effects of zymosan on antioxidation and immune function in S_(180) tumor-bearing mice, 70 Kunming mice were divided into 7 groups randomly. One group was the normal group, the others were divided into 6 groups randomly after S_(180) tumors-bearing mice mode was established by the inoculation of cancer cell suspension subcutaneously in mice's right anterior limbs.The mice were divided into low, medium and high dose zymosan groups, cyclophosphane(Cy) group, tumor control group and zymosan combination Cy group. At the 7th day S_(180) cancer cell suspension was transplanted in six groups, MDA,GSH-Px and SOD activity in liver homogenate at 19th day were analyzed to estimate the antioxidation effect of zymosan. RT-PCR(reverse transcription polymerase chain reaction)was used to detect the mRNA expression of IL-2,TNF-α and TGF-β_1 to appraise zymosan influence on S_(180) tumor-bearing mice immune function. Zymosan groups increased the activities of GSH-Px and SOD activity in the liver, only high dose zymosan groups reduced the levels of MDA(P＜0.01).The mRNA expression levels of S_(180) tumor-bearing mice tumor tissue TNF-α and IL-2 were higher obviously in the zymosan group than that in the Cy group and tumor control group(P＜0.01), and down-regulated level of TGF-β_1. The high dose zymosan conspicuously possessed a depressant effect on S_(180) tumor and joint action with combination Cy and enhancement of the mRNA expression of TNF-α, IL-2 of mice. The mechanism of tumor-inhibition by zymosan is mainly due to enhancing the immune and antioxidation function, and furthermore, the effect is dose dependent.