基础医学与临床
基礎醫學與臨床
기출의학여림상
BASIC MEDICAL SCIENCES AND CLINICS
2010年
2期
185-188
,共4页
葛璞%李春莉%冉亚林%叶彬
葛璞%李春莉%冉亞林%葉彬
갈박%리춘리%염아림%협빈
刚地弓形虫%人肺癌细胞A549%细胞周期
剛地弓形蟲%人肺癌細胞A549%細胞週期
강지궁형충%인폐암세포A549%세포주기
Toxoplasma gondii%lung cancer cell A549%cell cycle
目的 研究刚地弓形虫(Toxoplasma gondii)培养上清对体外培养的肺癌细胞A549增殖及细胞周期的影响.方法 取对数生长期的A549细胞(浓度为5×10~4mL~(-1))分别接种于不同细胞培养板,对照组加入RPMI-1640孵育,试验组加入相同体积不同数量(4×10~7 mL~(-1)、8×10~7mL~(-1)、16×10~7 mL~(-1))弓形虫速殖子培养上清孵育不同时间后,四甲基氮噻唑蓝(MTr)法检测吸光度(A_(490)值);PI染色后检测细胞周期;以Western blot检测细胞周期蛋白cyclinB1、cdc2表达或活性.结果 弓形虫培养上清呈时间剂量依赖性抑制A549细胞系增殖,处理组细胞周期在(G_2/M期产生阻滞;A549细胞系的cyclinB1、cdc2蛋白表达量下降.结论 刚地弓形虫培养上清能够抑制肺癌A549细胞系增殖,并通过调节cyclinB1、cdc2等蛋白表达或活性改变引起肺癌A549细胞系G_2/M期阻滞.
目的 研究剛地弓形蟲(Toxoplasma gondii)培養上清對體外培養的肺癌細胞A549增殖及細胞週期的影響.方法 取對數生長期的A549細胞(濃度為5×10~4mL~(-1))分彆接種于不同細胞培養闆,對照組加入RPMI-1640孵育,試驗組加入相同體積不同數量(4×10~7 mL~(-1)、8×10~7mL~(-1)、16×10~7 mL~(-1))弓形蟲速殖子培養上清孵育不同時間後,四甲基氮噻唑藍(MTr)法檢測吸光度(A_(490)值);PI染色後檢測細胞週期;以Western blot檢測細胞週期蛋白cyclinB1、cdc2錶達或活性.結果 弓形蟲培養上清呈時間劑量依賴性抑製A549細胞繫增殖,處理組細胞週期在(G_2/M期產生阻滯;A549細胞繫的cyclinB1、cdc2蛋白錶達量下降.結論 剛地弓形蟲培養上清能夠抑製肺癌A549細胞繫增殖,併通過調節cyclinB1、cdc2等蛋白錶達或活性改變引起肺癌A549細胞繫G_2/M期阻滯.
목적 연구강지궁형충(Toxoplasma gondii)배양상청대체외배양적폐암세포A549증식급세포주기적영향.방법 취대수생장기적A549세포(농도위5×10~4mL~(-1))분별접충우불동세포배양판,대조조가입RPMI-1640부육,시험조가입상동체적불동수량(4×10~7 mL~(-1)、8×10~7mL~(-1)、16×10~7 mL~(-1))궁형충속식자배양상청부육불동시간후,사갑기담새서람(MTr)법검측흡광도(A_(490)치);PI염색후검측세포주기;이Western blot검측세포주기단백cyclinB1、cdc2표체혹활성.결과 궁형충배양상청정시간제량의뢰성억제A549세포계증식,처리조세포주기재(G_2/M기산생조체;A549세포계적cyclinB1、cdc2단백표체량하강.결론 강지궁형충배양상청능구억제폐암A549세포계증식,병통과조절cyclinB1、cdc2등단백표체혹활성개변인기폐암A549세포계G_2/M기조체.
Objective To investigate whether there is an inhibition of the human lung cancer cell line A549 induced by the culture supernatant of Toxoplasma gondii in vitro and the mechanism of the inhibition. Methods A549 cells 5 x 10~4mL~(-1) were cultured and harvested. The cells were treated for different hours with different concentrations of Toxoplasma gondii culture supernatant (the concentrations of tachyzoites were 4×10~7mL~(-1), 8 × 10~7mL~(-1), 16 ×10~7mL~(-1) respectively). Growth inhibition rate was measured with the MTT method; Cell cycle was checked with flow cytometer. Western blot was used to detect the level of cyclinBl and cdc2 of cells. Results The culture supernatants of Toxoplasma gondii inhibited proliferation of A549 cells in a time-dose dependent manner. Cell cycle was significantly stopped at G_2/M phase by the culture supernatants with FCM technology. The culture supernatant of Toxoplasma gondii reduced the expressions of gene cyclinBl and cdc2 of A549 cells. Conclusion The culture supernatant of Toxoplasma gondii may inhibit A549 cell and arrest the cell cycle of A549 cells mainly by regulating the expression of gene cyclinBl and cdc2.
