CAJ | 학술논문

运用反相高效液相色谱(RP/HPLC)法对21种蕨类植物中槲皮素、山柰酚的含量进行测定.使用Shimadzu C_(18) (250 mm×4.6 mm,5 μm)色谱柱,以甲醇-水溶液为流动相进行等度洗脱,流速1.0 mL/min,检测波长360 nm,进样量20 μL,柱温28 ℃.各对照品的质量浓度与色谱峰面积线性关系良好,具有较好的精确度和重复性,槲皮素、山柰酚的加标回收率分别为93%和95%.采用该方法分别对采自秦岭的21种蕨类植物的地上和地下部分进行测定,地上部分有19种含槲皮素、15种含山柰酚,其中毡毛石韦中槲皮素含量最高(2.11 mg/g),蜈蚣草中山柰酚含量最高(19.80 mg/g);而地下部分除有边瓦韦、大瓦韦含槲皮素(含量分别为0.11、0.12 mg/g)外,其余根状茎中几乎没有这两种黄酮类化合物;表明槲皮素与山柰酚在蕨类植物的地上部分广泛存在.
운용반상고효액상색보(RP/HPLC)법대21충궐류식물중곡피소、산내분적함량진행측정.사용Shimadzu C_(18) (250 mm×4.6 mm,5 μm)색보주,이갑순-수용액위류동상진행등도세탈,류속1.0 mL/min,검측파장360 nm,진양량20 μL,주온28 ℃.각대조품적질량농도여색보봉면적선성관계량호,구유교호적정학도화중복성,곡피소、산내분적가표회수솔분별위93%화95%.채용해방법분별대채자진령적21충궐류식물적지상화지하부분진행측정,지상부분유19충함곡피소、15충함산내분,기중전모석위중곡피소함량최고(2.11 mg/g),오공초중산내분함량최고(19.80 mg/g);이지하부분제유변와위、대와위함곡피소(함량분별위0.11、0.12 mg/g)외,기여근상경중궤호몰유저량충황동류화합물;표명곡피소여산내분재궐류식물적지상부분엄범존재.
A reverse-phase high-performance liquid chromatographic(RP/HPLC)method was developed for simultaneous determination of quercetin and kaempferol in 21 ferns samples collected from Tsinling Mountains.The separation of the targets was performed on a Shimadzu C_(18) (250 mm×4.6 mm,5 μm) column by a constant gradient program using H2O(A) -methanol(B) as mobile phase at flow rate of 1.0 mL/min.The detection wavelength was set at 360 nm.The injection volume was 20 μL,and the column temperature was maintained at 28 ℃.The method showed good linear relationship,precision and repeatability.The recoveries were between 93% and 95%.The method was applied in the detection of quercetin and kaempferol in the above-ground parts and the underground parts of these 21 ferns.The result indicated that,in the above-ground parts,quercetin was existed in 19 species and kaempferol was existed in 15 species,in which the highest content of quercetin was 2.11 mg/g in Pyrrosia drakeana(Franch.) and that of kaempferol was 19.80 mg/g in Pteris vittata L.But in the underground parts of these 21 ferns,there was almost none of two kinds of elements in the rhizome of ferns except the existing of quercetin in Lepisorus marginatus and Lepisorus macrosphaerus(Baker) samples.The result also indicated that quercetin and kaempferol were widely existed in the above-ground parts of ferns samples.