光谱实验室
光譜實驗室
광보실험실
CHINESE JOURNAL OF SPECTROSCOPY LABORATORY
2010年
2期
602-606
,共5页
刘洲君%李启富%蒋袁娟%黄佳祎%成丹
劉洲君%李啟富%蔣袁娟%黃佳祎%成丹
류주군%리계부%장원연%황가의%성단
酶循环%荧光法%辅酶烟酰胺腺嘌呤二核苷酸%多功能微孔板检测系统
酶循環%熒光法%輔酶煙酰胺腺嘌呤二覈苷痠%多功能微孔闆檢測繫統
매순배%형광법%보매연선알선표령이핵감산%다공능미공판검측계통
Enzymatic Cycling%Fluorescence Method%NADH%Multifunction MPP Detection System
用酶循环荧光测定法,多功能微孔板检测系统检测人血清还原型烟酰胺腺嘌呤二核苷酸(NADH)的浓度.通过底物和酶的循环反应将待测物质NADH的量扩大至荧光可检测范围.该方法的荧光强度与NADH的质量在0.00-0.80pmol范围内呈良好的线性关系,建立的线性方程式为:y=99.377x+13.481,r=0.9941,加标回收率为95.5%-100.0%.批内RSD为1.0%-2.7%.批间RSD为1.9%-12.6%.该方法准确度、灵敏度高、稳定性好,可用于NADH的定量检测.
用酶循環熒光測定法,多功能微孔闆檢測繫統檢測人血清還原型煙酰胺腺嘌呤二覈苷痠(NADH)的濃度.通過底物和酶的循環反應將待測物質NADH的量擴大至熒光可檢測範圍.該方法的熒光彊度與NADH的質量在0.00-0.80pmol範圍內呈良好的線性關繫,建立的線性方程式為:y=99.377x+13.481,r=0.9941,加標迴收率為95.5%-100.0%.批內RSD為1.0%-2.7%.批間RSD為1.9%-12.6%.該方法準確度、靈敏度高、穩定性好,可用于NADH的定量檢測.
용매순배형광측정법,다공능미공판검측계통검측인혈청환원형연선알선표령이핵감산(NADH)적농도.통과저물화매적순배반응장대측물질NADH적량확대지형광가검측범위.해방법적형광강도여NADH적질량재0.00-0.80pmol범위내정량호적선성관계,건립적선성방정식위:y=99.377x+13.481,r=0.9941,가표회수솔위95.5%-100.0%.비내RSD위1.0%-2.7%.비간RSD위1.9%-12.6%.해방법준학도、령민도고、은정성호,가용우NADH적정량검측.
Enzyme cycling fluorimetry was applied to determine the concentration of reduced form of nicotinamide-adenine dinucleotid (NADH) in human red blood cells using muhifunction MPP detection system.After circular reaction of the substrates and enzymes, the concentration of NADH arrived at the tested region.The calibration graph for the determination of NADH is in the range of 0.00-0.80pmol, with linear equation of y = 99.377x + 13.481, r = 0.9941.The average recovery is 95.5%-100.0%,intra assay RSD is 1.0%-2.7% and inter assay RSD is 1.9%-12.6%.The method is accurate,sensitive and steady.
