CAJ | 학술논문

阿莫西林和多四环素在紫外光谱区间吸收峰附近有多重共线现象,紫外分光光度法难以准确测出2种物质各自含量.本文通过L_(16)(4~5)正交实验设计,采集200～250 nm波长混合样品吸收光谱,数据经标准化处理,建立偏最小二乘回归预报数学模型.结果显示,该法计算阿莫西林和多四环素的预测相对误差(RPE)分别为3.13%和6.42%,平均加标回收率分别为100.34%和100.71%.该法简便快捷,准确可靠,样品无需预先分离.
아막서림화다사배소재자외광보구간흡수봉부근유다중공선현상,자외분광광도법난이준학측출2충물질각자함량.본문통과L_(16)(4~5)정교실험설계,채집200～250 nm파장혼합양품흡수광보,수거경표준화처리,건립편최소이승회귀예보수학모형.결과현시,해법계산아막서림화다사배소적예측상대오차(RPE)분별위3.13%화6.42%,평균가표회수솔분별위100.34%화100.71%.해법간편쾌첩,준학가고,양품무수예선분리.
The existence of colinear and overlapping phenomena in the ultraviolet absorption spectra of amoxicillin and doxycycline makes it difficult to use ultraviolet spectrophotometry to determine the respective contents in a mixture of both components.L_(16)(4~5) orthogonal experimental design was applied in this study and absorption data from of 200 to 250 nm of their mixture were obtained with UV scan procedure.Then a data normalization was made and a PLS (partial least square regression) predicting model was developed by using DPS 8.01 software.It was shown that this method was accurate and reliable,with the RPE% for both amoxicillin and doxycycline were 3.13% and 6.42%,respectively,and the average recovery rate were 100.34% and 100.71%,respectively.It was also simple and rapid without the necessity of separating the samples beforehand.