中国综合临床
中國綜閤臨床
중국종합림상
CLINICAL MEDICINE OF CHINA
2012年
11期
1131-1134
,共4页
叶静华%蔡小燕%罗敏%林小军%唐莼%李伟念
葉靜華%蔡小燕%囉敏%林小軍%唐莼%李偉唸
협정화%채소연%라민%림소군%당순%리위념
类风湿关节炎%Th17细胞%流式细胞术
類風濕關節炎%Th17細胞%流式細胞術
류풍습관절염%Th17세포%류식세포술
Rheumatoid arthritis%Th17 cells%Flow cytometry
目的 探讨Th17细胞在类风湿关节炎(RA)患者外周血中的表达及临床意义.方法 选取活动期RA患者33例(其中合并骨侵蚀19例)、稳定期RA患者27例及健康对照组30例为研究对象.运用流式细胞术胞内细胞因子染色的方法检测研究对象外周血Th17细胞,并进一步分析其与疾病活动性(DAS28评分)和骨侵蚀的关系.结果 60例RA患者外周血Th17细胞表达率为(1.60±0.51)％,健康对照组为(0.81±0.21)％,差异有统计学意义(t=10.30,P＜0.01).其中,活动期RA患者外周血Th17细胞的表达率为(1.94±0.38)％,显著高于稳定期RA患者[(1.19±0.27)％]和健康对照组(t值分别为8.85和14.60,P均＜0.01),而稳定期RA患者外周血Th17细胞的表达率亦高于健康对照组(=5.88,P＜0.01).在活动期RA患者中,19例存在骨侵蚀的患者外周血Th17细胞的表达率显著高于14例无骨侵蚀的患者[(2.09±0.39)％与(1.74±0.29)％,t=2.83,P＜0.01].RA患者外周血Th17细胞的表达与DAS28评分呈正相关(r=0.86,P＜0.01).结论 RA患者外周血有高水平的Th17细胞表达,并与疾病活动性和骨破坏相关.Th17细胞可能在RA发病及病情发展中起重要作用.
目的 探討Th17細胞在類風濕關節炎(RA)患者外週血中的錶達及臨床意義.方法 選取活動期RA患者33例(其中閤併骨侵蝕19例)、穩定期RA患者27例及健康對照組30例為研究對象.運用流式細胞術胞內細胞因子染色的方法檢測研究對象外週血Th17細胞,併進一步分析其與疾病活動性(DAS28評分)和骨侵蝕的關繫.結果 60例RA患者外週血Th17細胞錶達率為(1.60±0.51)％,健康對照組為(0.81±0.21)％,差異有統計學意義(t=10.30,P＜0.01).其中,活動期RA患者外週血Th17細胞的錶達率為(1.94±0.38)％,顯著高于穩定期RA患者[(1.19±0.27)％]和健康對照組(t值分彆為8.85和14.60,P均＜0.01),而穩定期RA患者外週血Th17細胞的錶達率亦高于健康對照組(=5.88,P＜0.01).在活動期RA患者中,19例存在骨侵蝕的患者外週血Th17細胞的錶達率顯著高于14例無骨侵蝕的患者[(2.09±0.39)％與(1.74±0.29)％,t=2.83,P＜0.01].RA患者外週血Th17細胞的錶達與DAS28評分呈正相關(r=0.86,P＜0.01).結論 RA患者外週血有高水平的Th17細胞錶達,併與疾病活動性和骨破壞相關.Th17細胞可能在RA髮病及病情髮展中起重要作用.
목적 탐토Th17세포재류풍습관절염(RA)환자외주혈중적표체급림상의의.방법 선취활동기RA환자33례(기중합병골침식19례)、은정기RA환자27례급건강대조조30례위연구대상.운용류식세포술포내세포인자염색적방법검측연구대상외주혈Th17세포,병진일보분석기여질병활동성(DAS28평분)화골침식적관계.결과 60례RA환자외주혈Th17세포표체솔위(1.60±0.51)％,건강대조조위(0.81±0.21)％,차이유통계학의의(t=10.30,P＜0.01).기중,활동기RA환자외주혈Th17세포적표체솔위(1.94±0.38)％,현저고우은정기RA환자[(1.19±0.27)％]화건강대조조(t치분별위8.85화14.60,P균＜0.01),이은정기RA환자외주혈Th17세포적표체솔역고우건강대조조(=5.88,P＜0.01).재활동기RA환자중,19례존재골침식적환자외주혈Th17세포적표체솔현저고우14례무골침식적환자[(2.09±0.39)％여(1.74±0.29)％,t=2.83,P＜0.01].RA환자외주혈Th17세포적표체여DAS28평분정정상관(r=0.86,P＜0.01).결론 RA환자외주혈유고수평적Th17세포표체,병여질병활동성화골파배상관.Th17세포가능재RA발병급병정발전중기중요작용.
Objective To investigate the expression and significance of Thl7 cells in peripheral blood of patients with Rheumatoid Arthritis (RA).Methods Thirty-three active RA and 27 stable RA patients and 30 healthy individuals were enrolled in the study.Intracellular flow cytometry detection of TH17 cells in peripheral blood was established.The correlations between Th17 cells and RA disease activity (DAS28 scale) as well as bone erosion were analyzed.Results The expressions of Th17 cells in the patients with RA were significantly higher than those of healthy controls [ ( 1.60 ± 0.51 ) ％ vs (0.81 ± 0.21 ) ％,t =10.30,P ＜ 0.001 ].The Th17 levels in active RA patients were also higher than that of stable RA patients and healthy subjects [ ( 1.94 ±0.38 ) ％ vs ( 1.19 ± 0.27) ％,t =8.85,14.60,P ＜ 0.01 ].And the Th1 7 levels in stable RA patients were also higher than that of healthy subjects (t =5.88,P ＜0.01 ).Active RA patients with bone erosion ( 19 cases) also had higher levels of Th17 cells than those without bone erosion ( 14 cases) [ ( 2.09 ± 0.39 ) ％ vs.( 1.74 ±0.29 ) ％,t =2.83,P ＜ 0.01 ].The expression of Th17 cells was positively correlated with the DAS28 score ( r =0.86,P ＜ 0.01 ).Conclusion There is a high expression of Th17 cells in peripheral blood of RA patients,which is related to disease activity and bone erosion.Th17 cells might play an important role in the pathogenesis of RA.
