CAJ | 학술논문

目的探讨人早孕蜕膜组织是否存在干细胞亚群(SP细胞)及其生物学特性.方法取孕早期人流后子宫蜕膜组织,酶消化法获得组织细胞悬液,经Hoeehst33342染色或与维拉帕米共染后,采用免疫荧光细胞分选法(FACS)鉴定和分选干细胞,并以条件培养基培养观察细胞的增殖和集落生成能力.结果孕早期子宫蜕膜组织中存在SP干细胞,其平均含量为(0.8±0.7)%,且在体外长期培养后有细胞增殖和形成集落.不同孕周间SP干细胞含量差异无统计学意义(P>0.05).结论孕早期子宫蜕膜组织中存在SP干细胞,并具有细胞增殖和集落生成能力.
목적 탐토인조잉세막조직시부존재간세포아군(SP세포)급기생물학특성.방법 취잉조기인류후자궁세막조직,매소화법획득조직세포현액,경Hoeehst33342염색혹여유랍파미공염후,채용면역형광세포분선법(FACS)감정화분선간세포,병이조건배양기배양관찰세포적증식화집락생성능력.결과 잉조기자궁세막조직중존재SP간세포,기평균함량위(0.8±0.7)%,차재체외장기배양후유세포증식화형성집락.불동잉주간SP간세포함량차이무통계학의의(P>0.05).결론 잉조기자궁세막조직중존재SP간세포,병구유세포증식화집락생성능력.
Objective To explore the existence of side-population cells (SP cells) in human decidual tissues in early pregnancy, and its biological characteristics. Methods Decidual cells were obtained by enzymatic digestion from the decidual tissues of human early pregnancy. The cells then were stained with Hoechst33342 dye either alone or in combination with verapamil. Fluorescence-activated cell sorter (FACS) analysis was used to identify and isolate SP cells. The isolated cells were cultured in conditioned media culture to observe the proliferation and ability to form colony. Results Stem cells were found in the decidual tissues of early pregnancy with a percentage of 0.78%± 0.71% (0% -3.20% ). These cells further proliferated and formed colonies during long-time culture in vitro. No significant statistical difference was found in contents of SP cells among different gestational week (P >0.05 ). Conclusion SP cells exist in human decidua that can further proliferate and have the clonogenicity in culture in vitro.