CAJ | 학술논문

目的研究Abil在肝细胞癌(HCC)组织中的表达情况及其临床意义.方法采用RT-PCR法检测40例HCC组织及相应癌旁肝组织标本中Abil mRNA的表达情况;免疫组织化学法检测加例HCC组织及相应癌旁肝组织标本中Abil蛋白的表达情况;结合临床病理及随访资料分析Abil表达水平与HCC侵袭转移及预后的关系.结果 Abil mRNA在HCC组织中的表达水平高于相应的癌旁组织(1.32±0.75比0.74±0.52,P<0.05);Abil mRNA在结节性肝癌组织中的表达水平亦明显高于孤立性大肝癌组织(1.72±0.83比1.14±0.57,P<0.05).Abil蛋白主要表达于HCC细胞的胞质内,其表达水平与肿瘤结节数目、是否有包膜、静脉癌栓及Edmondson-Steiner分级密切相关(P<0.05),而与HCC患者的性别、有无肝硬化及肿瘤直径无明显相关性(P>0.05).Abil蛋白高表达组HCC患者的复发转移率明显高于低表达组患者(P<0.05);生存率则明显低于低表达组患者(P<0.02).结论 Abil在HCC组织中的表达水平较癌旁组织高,其表达水平与HCC的结节数目、是否有包膜、静脉癌栓、Edmondson-Steiner分级及临床预后密切相关.
목적 연구Abil재간세포암(HCC)조직중적표체정황급기림상의의.방법 채용RT-PCR법검측40례HCC조직급상응암방간조직표본중Abil mRNA적표체정황;면역조직화학법검측가례HCC조직급상응암방간조직표본중Abil단백적표체정황;결합림상병리급수방자료분석Abil표체수평여HCC침습전이급예후적관계.결과 Abil mRNA재HCC조직중적표체수평고우상응적암방조직(1.32±0.75비0.74±0.52,P<0.05);Abil mRNA재결절성간암조직중적표체수평역명현고우고립성대간암조직(1.72±0.83비1.14±0.57,P<0.05).Abil단백주요표체우HCC세포적포질내,기표체수평여종류결절수목、시부유포막、정맥암전급Edmondson-Steiner분급밀절상관(P<0.05),이여HCC환자적성별、유무간경화급종류직경무명현상관성(P>0.05).Abil단백고표체조HCC환자적복발전이솔명현고우저표체조환자(P<0.05);생존솔칙명현저우저표체조환자(P<0.02).결론 Abil재HCC조직중적표체수평교암방조직고,기표체수평여HCC적결절수목、시부유포막、정맥암전、Edmondson-Steiner분급급림상예후밀절상관.
Objective To investigate the expression of Abil in hepatocellular carcinama (HCC)and the correlation between its expression level and clinical pathological characteristies as well as prognosis of HCC.Methods Abil expression was determined at both mRNA and protein levels in 40 HCC tissues and their corresponding paracancinomatous liver tissues by RT-PCR and immunohistochemistry.The correlations between Abil expression levels and pathological characteristics as well as prognosis were also analyzed.Results The expression level of Abil mRNA in HCC tissues were significantly higher than those in corresponding paracarcinomatous liver tissue (P<0.05),and the expression level of Abil mRNA in nodular HCC tissues were also significantly higher than those in solitary large HCC tissues.Immunohistochemistry results showed that Abil protein located in cytoplasm of HCC cells and the expression level of Abil protein were significant positive correlated with the number of HCC,capsular formation,venous invasion and Edmondson-Steiner grade (P<0.05).Combined with follow-up data,the results also showed that HCC patients with high Abil protein expression had a higher risk of invasion/metastasis and a shorter survival than those with low Abil protein expression (P<0.05).Conclusions Expression level of Abil is up-regulated in HCC tissues compared with corresponding paracarcinomatous liver tissue and the expression level of Abil is significantly correlated with the number of tumor,capsular formation,venous invasion,Edmondson-Steiner grade and prognosis of HCC.