林业科学
林業科學
임업과학
SCIENTIA SILVAE SINICAE
2005年
1期
205-210
,共6页
高克祥%刘晓光%Dana Friesem%Leonid Chernin%时呈奎
高剋祥%劉曉光%Dana Friesem%Leonid Chernin%時呈奎
고극상%류효광%Dana Friesem%Leonid Chernin%시정규
螺旋毛壳ND35%细胞壁降解酶%活性检测%重寄生%扫描电镜
螺鏇毛殼ND35%細胞壁降解酶%活性檢測%重寄生%掃描電鏡
라선모각ND35%세포벽강해매%활성검측%중기생%소묘전경
Chaetomium spirale ND35%cell wall degrading enzymes%assays of enzyme activities%mycoparasitism%scanning electron microscope
Some Chaetomium spp. Are capable of antagonizing several plant pathogenic fungi through production of antibiotics and mycoparasitism. Secretion of lytic enzymes, mainly including glucanases and chitinases, is considered the most important step in the mycoparasitic process. In this study, an about 110kDa exo - β - 1,3 - glucanase from C. Spirale ND35 was detected both in culture filtrate and directly on PAGE and IEF gels, as well as chitinases, although protease was not detectable on Litmus milk agar plates. Coiling and penetrating the hyphae of host fungus Valsa mali were observed by scanning electron microscope (SEM), which may be related to the synergistic interaction between β - 1,3 - glucanase and chitinases. Β - 1,3 - glucanase activity of C. Spirale ND35 varied considerably when C. Spirale ND35 was grown in different carbon sources during various incubation time, and might be subjected to both induction by substrate and catabolite repression.
