中国实验血液学杂志
中國實驗血液學雜誌
중국실험혈액학잡지
JOURNAL OF EXPERIMENTAL HEMATOLOGY
2009年
1期
60-64
,共5页
吴亚男%陈宝安%程坚%高峰%许文林%丁家华%高冲%孙新臣%李国宏%陈文姬%刘莉洁%李小毛%王雪梅
吳亞男%陳寶安%程堅%高峰%許文林%丁傢華%高遲%孫新臣%李國宏%陳文姬%劉莉潔%李小毛%王雪梅
오아남%진보안%정견%고봉%허문림%정가화%고충%손신신%리국굉%진문희%류리길%리소모%왕설매
5溴汉防己甲素%磁性纳米四氧化三铁颗粒%柔红霉素%多药耐药%移植瘤模型%白血病
5溴漢防己甲素%磁性納米四氧化三鐵顆粒%柔紅黴素%多藥耐藥%移植瘤模型%白血病
5추한방기갑소%자성납미사양화삼철과립%유홍매소%다약내약%이식류모형%백혈병
5-bromotetrandrine%magnetic nanoparticle of Fe3O4%daunorubicin%multidrug-resistance%xenograft model%leukemia
本研究探讨5溴汉防己甲素(5-Brlret)、磁性纳米Fe3O4(Fe3O4-MNP)协同化疗药在动物模型体内逆转耐药的作用及其作用机理.建立恶性血液病荷瘤鼠模型,将其随机分为5组.A组:生理盐水;B组:单用DNR;C组:5-BrTet复合DNR;D组:Fe3O4-MNPs复合DNR;E组:Fe3O4-MNPs复合DNR及5-BrTet.观察各组肿瘤生长特征,肿瘤重量、体积.取各组肿瘤以及心、肝、肾等脏器行组织病理观察.Western blot检测耐药肿瘤BCL-2,BAX,以及caspase-3表达.结果表明:对于K562移植瘤,B、C、D、E 4个组之间肿瘤抑制率没有明显的差别.对于K562/A02移植瘤,Fe3O4-MNP复合DNR及5-BrTet可明显抑制移植瘤的增殖,促进肿瘤细胞凋亡;肿瘤组织病理观察显示肿瘤坏死明显.5-BrTet联合Fe3O4-MNP抑制K562/A02移植瘤的BCL-2蛋白的表达,下调BAX和caspas-3的表达.结论:在耐药肿瘤动物模型体内,Fe3O4-MNPs协同DNR和5-BrTet具有很强的肿瘤抑制作用.
本研究探討5溴漢防己甲素(5-Brlret)、磁性納米Fe3O4(Fe3O4-MNP)協同化療藥在動物模型體內逆轉耐藥的作用及其作用機理.建立噁性血液病荷瘤鼠模型,將其隨機分為5組.A組:生理鹽水;B組:單用DNR;C組:5-BrTet複閤DNR;D組:Fe3O4-MNPs複閤DNR;E組:Fe3O4-MNPs複閤DNR及5-BrTet.觀察各組腫瘤生長特徵,腫瘤重量、體積.取各組腫瘤以及心、肝、腎等髒器行組織病理觀察.Western blot檢測耐藥腫瘤BCL-2,BAX,以及caspase-3錶達.結果錶明:對于K562移植瘤,B、C、D、E 4箇組之間腫瘤抑製率沒有明顯的差彆.對于K562/A02移植瘤,Fe3O4-MNP複閤DNR及5-BrTet可明顯抑製移植瘤的增殖,促進腫瘤細胞凋亡;腫瘤組織病理觀察顯示腫瘤壞死明顯.5-BrTet聯閤Fe3O4-MNP抑製K562/A02移植瘤的BCL-2蛋白的錶達,下調BAX和caspas-3的錶達.結論:在耐藥腫瘤動物模型體內,Fe3O4-MNPs協同DNR和5-BrTet具有很彊的腫瘤抑製作用.
본연구탐토5추한방기갑소(5-Brlret)、자성납미Fe3O4(Fe3O4-MNP)협동화료약재동물모형체내역전내약적작용급기작용궤리.건립악성혈액병하류서모형,장기수궤분위5조.A조:생리염수;B조:단용DNR;C조:5-BrTet복합DNR;D조:Fe3O4-MNPs복합DNR;E조:Fe3O4-MNPs복합DNR급5-BrTet.관찰각조종류생장특정,종류중량、체적.취각조종류이급심、간、신등장기행조직병리관찰.Western blot검측내약종류BCL-2,BAX,이급caspase-3표체.결과표명:대우K562이식류,B、C、D、E 4개조지간종류억제솔몰유명현적차별.대우K562/A02이식류,Fe3O4-MNP복합DNR급5-BrTet가명현억제이식류적증식,촉진종류세포조망;종류조직병리관찰현시종류배사명현.5-BrTet연합Fe3O4-MNP억제K562/A02이식류적BCL-2단백적표체,하조BAX화caspas-3적표체.결론:재내약종류동물모형체내,Fe3O4-MNPs협동DNR화5-BrTet구유흔강적종류억제작용.
This study was aimed to investigate the reversal effect of 5-bromotetrandrine(5-BrTet)and magnetic na noparticle of Fe3O4(Fe3O4-MNPs)combined with DNR in vivo.The xenograft leukemia model with stable multiple drug resistance in nude mice was established.The two sub clones of K562 and K562/A02 cells were respectively inocu 1ated subcutaneously into back of athymic nude mice(1×107 cells/each)to establish the leukemia xenografl models. Drug resistant and the sensitive tumor-bearing nude mice were both assigned randomly into 5 groups:group A was trea ted with NS:group B was treated with DNR;group C was treated with nanoparticle of Fe3O4 combined with DNR; group D was treated with 5-BrTet combined with DNR:group E was treated with 5-bromotetrandrine and magnetic nano particle of Fe3O4 combined with DNR.The incidence of tumor formation,growth characteristics,weight and volume of tumor were observed.The histopathologic examination of tumors and organs were carried out.The protein levels of BCL-2.BAX.and Caspase-3 in resistant tumors were detected by Western blot.The results indicated that 5-BrTet and magnetic nanoparticle of Fe3O4 combined with DNR significantly suppressed growth of K562/A02 cell xenogafi tumor, histopathologic examination of tumors showed the tumors necrosis obviously.Application of 5-BrTet and magnetic nano particle of Fe3O4 inhibited the expression of BCL-2 protein and up regulated the expression of BAX,and Caspase-3 pro tein in K562/A02 cell xenograft tumor.It is concluded that 5-bromotetrandrine and magnetic nanoparticle of Fe3O4 com bined wim DNR have significant tumor-suppressing effect on MDR leukemia cell xenograff model.
