CAJ | 학술논문

从采集的多种土壤样品中.获得了19株在3种筛选平板上均生长良好的候选氯氰菊酯降解菌.通过生理生化特性鉴定和16S rDNA序列的测定及比对.将其中的氯氰菊酯降解菌CY22-7鉴定为中华根瘤菌属(Sinorhizobium sp.).同时,对氯氰菊酯降解菌CY22-7的降解特性研究结果表明:(1)氯氰菊酯降解菌CY22-7以5%(体积分数)的接种鼍接种到氯氰菊酯起始质量浓度为100 mg/L的氯氰菊酯乙醇培养基后,于200 r/min、30℃摇床中培养.经过6 d的培养,氯氰菊酯降解菌CY22-7降解了约60%的氯氰菊酯.(2)加人外源营养物质有利于促进氯氰菊酯的降解.其中,葡萄糖和酵母提取物的促进作用最为明显.(3)氯氰菊酯降解菌CY22-7降解氯氰菊酯的最适温度为30℃,最佳pH为6.0.
종채집적다충토양양품중.획득료19주재3충사선평판상균생장량호적후선록청국지강해균.통과생리생화특성감정화16S rDNA서렬적측정급비대.장기중적록청국지강해균CY22-7감정위중화근류균속(Sinorhizobium sp.).동시,대록청국지강해균CY22-7적강해특성연구결과표명:(1)록청국지강해균CY22-7이5%(체적분수)적접충타접충도록청국지기시질량농도위100 mg/L적록청국지을순배양기후,우200 r/min、30℃요상중배양.경과6 d적배양,록청국지강해균CY22-7강해료약60%적록청국지.(2)가인외원영양물질유리우촉진록청국지적강해.기중,포도당화효모제취물적촉진작용최위명현.(3)록청국지강해균CY22-7강해록청국지적최괄온도위30℃,최가pH위6.0.
A cypermethrin-degrading bacterium named CY22-7 was isolated from pesticide contaminated soils. This bacterium was identified as Sinorhizobium sp. according to its physiological and biochemical characteristics and the similarity analysis of its 16S rDNA sequence. Under the inorganic salt medium with the bacterium inoculation of 5% ,60% of 100 mg/L cypermethrin was degraded by this strain in 6 d at 30℃. The effects of initial pesticide con-centration,added nutriment, temperature, and pH on cypermethrin degrading by CY22-7 was also investigated. The presence of glucose and yeast extract were beneficial for cypermethrin degrading.