CAJ | 학술논문

建立了一种毛细管电泳-电化学发光间接测定人血浆中盐酸甲氯芬酯(MFX)的新方法.在碱性条件下,三联吡啶钌在铂电极上的弱电化学发光信号可以被MFX增敏,且MFX水解后灵敏度比未水解的灵敏度提高了6倍.研究了工作电极电位、磷酸盐缓冲溶液浓度及其pH值、分离电压、进样电压和进样时间等实验参数对MFX水解产物N,N-二甲氨基乙醇测定的影响.在优化实验条件下,其浓度线性范围为0.020～50 μg/mL,检出限(3σ)为6.3 ng/mL,峰高的相对标准偏差为3.1%(10 μg/mL MFX,n=11).利用该法间接地测定了人血浆中MFX的含量,回收率为95.9%～96.9%.
건립료일충모세관전영-전화학발광간접측정인혈장중염산갑록분지(MFX)적신방법.재감성조건하,삼련필정조재박전겁상적약전화학발광신호가이피MFX증민,차MFX수해후령민도비미수해적령민도제고료6배.연구료공작전겁전위、린산염완충용액농도급기pH치、분리전압、진양전압화진양시간등실험삼수대MFX수해산물N,N-이갑안기을순측정적영향.재우화실험조건하,기농도선성범위위0.020～50 μg/mL,검출한(3σ)위6.3 ng/mL,봉고적상대표준편차위3.1%(10 μg/mL MFX,n=11).이용해법간접지측정료인혈장중MFX적함량,회수솔위95.9%～96.9%.
An indirect determination of meclofenoxate hydrochloride (MFX) in human plasma was developed by using capillary electrophoresis coupled with electrochemiluminescence (ECL) detection. The ECL intensity was obviously increased by hydrolysis product of MFX. The effects of different NaOH concentrations on hydrolysis ratios were examined and the operating parameters that affected the separation and detection were optimized. Under the optimal conditions, the linear range for MFX was from 0.01 to 50 μg/mL (r~2=0.9996) with a detection limit of 6.3 ng/mL (3σ). The method was successfully applied for the determination of meclofenoxate hydrochloride in human plasma with the recoveries varying from 95.9 to 96.9%.