CAJ | 학술논문

[目的]定位粳稻农艺性状对外源赤霉素敏感性的QTL,为选育和改良高敏感性不育系提供理论依据.[方法]以粳稻秀水79与C堡及其杂交后代衍生的重组自交系群体260 个株系为研究材料,研究其4 个农艺性状对外源赤霉素处理的敏感性并对其QTL进行定位.敏感性用反应指数衡量,反应指数越大,敏感性越高.采用Win QTL Cartographer 2.5软件的复合区间作图法(CIM),在南京农业大学作物遗传与种质创新国家重点实验室构建的粳稻SSR分子标记连锁图谱的17 个连锁群上每隔2 cm计算LOD值.采用排列组合1 000 次方法确定LOD阈值,以保证全基因组检测到的QTL犯Ⅰ类错误概率小于5%.当实际求得的LOD值大于LOD阈值时,就认为该区段存在1个QTL,其置信区间为LOD峰值向下1 个LOD值单位区间.QTL命名遵循McCouch等规则.估算每个QTL的贡献率和加性效应.[结果]控制剑叶角度对赤霉素敏感性的QTL共有3 个,分别位于第3、9、9染色体上,其贡献率分别为5.59%,13.00%和11.80%,增效等位基因分别来自秀水79,C堡和C堡;控制株高对赤霉素敏感性的QTL共有2 个,分别位于第1和8染色体上,其贡献率分别为8.46%和10.97%,增效等位基因分别来自C堡和秀水79;控制第1节间长度对赤霉素敏感性的QTL只有1 个,位于第3染色体上,其贡献率为0.05%,增效等位基因来自C堡;控制第2节间长度对赤霉素敏感性的QTL也只有1 个,位于第1染色体上,其贡献率为7.34%,增效等位基因来自C堡.[结论]该研究结果对减少杂交水稻制种过程中外源赤霉素的使用量、节约制种成本、降低对环境的污染具有重要意义.
[목적]정위갱도농예성상대외원적매소민감성적QTL,위선육화개량고민감성불육계제공이론의거.[방법]이갱도수수79여C보급기잡교후대연생적중조자교계군체260 개주계위연구재료,연구기4 개농예성상대외원적매소처리적민감성병대기QTL진행정위.민감성용반응지수형량,반응지수월대,민감성월고.채용Win QTL Cartographer 2.5연건적복합구간작도법(CIM),재남경농업대학작물유전여충질창신국가중점실험실구건적갱도SSR분자표기련쇄도보적17 개련쇄군상매격2 cm계산LOD치.채용배렬조합1 000 차방법학정LOD역치,이보증전기인조검측도적QTL범Ⅰ류착오개솔소우5%.당실제구득적LOD치대우LOD역치시,취인위해구단존재1개QTL,기치신구간위LOD봉치향하1 개LOD치단위구간.QTL명명준순McCouch등규칙.고산매개QTL적공헌솔화가성효응.[결과]공제검협각도대적매소민감성적QTL공유3 개,분별위우제3、9、9염색체상,기공헌솔분별위5.59%,13.00%화11.80%,증효등위기인분별래자수수79,C보화C보;공제주고대적매소민감성적QTL공유2 개,분별위우제1화8염색체상,기공헌솔분별위8.46%화10.97%,증효등위기인분별래자C보화수수79;공제제1절간장도대적매소민감성적QTL지유1 개,위우제3염색체상,기공헌솔위0.05%,증효등위기인래자C보;공제제2절간장도대적매소민감성적QTL야지유1 개,위우제1염색체상,기공헌솔위7.34%,증효등위기인래자C보.[결론]해연구결과대감소잡교수도제충과정중외원적매소적사용량、절약제충성본、강저대배경적오염구유중요의의.
[Objective] The research aimed to map QTL for the sensitivity of the traits related outcrosssing of Japonica rice to exogenous GA3 and provide theoretical basis for breeding and improving the high-sensitivity sterile line. [Method] Taking Japonica rice Xiushui 79 and C bao and their recombinant inbred line population 260 lines as test materials, the sensitivity of 4 traits related outcrossing to Exogenous GA3 and their QTL mapping were studied by using composite interval mapping.. [Result]Three QTLs, which controlled the sensitivity of flag leaf angle to GA3, were detected on chromosome 3, 9 and 9, and they explained 5.6%,13% and 11.8% of phenotypic variance, respectively. Positive alleles came from Xiushui 79, C bao and C bao, respectively. Two QTLs, which controlled the sensitivity of plant height to GA3, were detected on chromosome 1 and 8, they explained 8.46% and 11% of phenotypic variance, respectively. Positive alleles came from Xiushui 79, and C bao, respectively. One QTL, which controlled the sensitivity of the first internode length to GA3, was detected on chromosome 3, and it explained 0.05% of phenotypic variance. Positive alleles came from C bao. One QTL, which controlled the sensitivity of the second internode length to GA3, was detected on chromosome 1, and it explained 7.34% of phenotypic variance. Positive alleles came from C bao. [Conclusion] The research results had important seed production cost, reducing the pollution of the environment.