CAJ | 학술논문

[目的]建立一种较为简单且有效的香蕉种属关系鉴定的手段和方法.[方法]根据不同香蕉品种线粒体基因内含子序列间的差异性,通过PCR扩增线粒体基因组中细胞色素氧化酶亚基II基因中的一个内含子,并进行测序和聚类分析,对分属5个基因型 (AAA、AA、AAB、ABB和BB)的16个香蕉品种进行了分类.[结果]16个香蕉品种可分为三大类:第一类包括1个品种,基因型为BB;第二类包括7个品种,基因型为ABB;第三类包括8个品种,基因型包括AA、AAA、AAB和BB.其中,抗病新品种逸仙1、2、3号与粉杂有最近的亲缘关系.[结论]该方法对香蕉品种的分类结果与基因型的组成类型基本一致,说明它在鉴定香蕉种属亲缘关系中是可行和有效的.
[목적]건립일충교위간단차유효적향초충속관계감정적수단화방법.[방법]근거불동향초품충선립체기인내함자서렬간적차이성,통과PCR확증선립체기인조중세포색소양화매아기II기인중적일개내함자,병진행측서화취류분석,대분속5개기인형 (AAA、AA、AAB、ABB화BB)적16개향초품충진행료분류.[결과]16개향초품충가분위삼대류:제일류포괄1개품충,기인 형위BB;제이류포괄7개품충,기인형위ABB;제삼류포괄8개품충,기인형포괄AA、AAA、AAB화BB.기중,항병신품충일선1、2、3호여분잡유최근적친연관계.[결론]해방법대향초품충적분류결과여기인형적조성류형기본일치,설명타재감정향초충속친연관계중시가행화유효적.
[Objective] The aim was to establish an effective method for the identification of genetic relationships among different varieties in Musa paradisaca. [Method] Based on the diversity of mitochondria DNA intron sequence among different varieties of M. paradisaca,an intron of cytochrome oxidase subunit II gene in mitochondria DNA genome was amplified and sequenced. And then the cluster analysis was used to classify 16 varieties of M. paradisaca,which belonged to five genotypes (AAA,AA,AAB,ABB and BB). [Result] The 16 varieties of M. paradisaca could be divided to three classes: the first class contained one variety,the genotype of which was BB; the second class contained seven varieties,the genotype of which was ABB; the third class contained eight varieties,the genotypes of which included AA,AAA,AAB and BB. The new varieties YiXian 1,2 and 3 showed the nearest relationship with FenZa. [Conclusion] The result of classification was consistent with the genotypes,thus verified the feasibility and effectiveness of the new method in the genetic relationship identification of M. paradisaca germplasm.