中华胃肠外科杂志
中華胃腸外科雜誌
중화위장외과잡지
CHINESE JOURNAL OF GASTROINTESTINAL SURGERY
2011年
5期
368-371
,共4页
束平%秦净%秦新裕%孙益红%沈振斌%王燕娜%刘景磊
束平%秦淨%秦新裕%孫益紅%瀋振斌%王燕娜%劉景磊
속평%진정%진신유%손익홍%침진빈%왕연나%류경뢰
胃肿瘤%CD163抗原%巨噬细胞%Foxp3%调节性T细胞
胃腫瘤%CD163抗原%巨噬細胞%Foxp3%調節性T細胞
위종류%CD163항원%거서세포%Foxp3%조절성T세포
Stomach neoplasms%CD163 antigen%Macrophages%Foxp3%Regulatory T cells
目的 探讨胃癌组织中M2型巨噬细胞和调节性T细胞(Tregs)的表达情况及其联合检测对预后的预测价值.方法 收集1999年2月至2005年12月期间复旦大学附属中山医院行D2根治术并达到R0切除的135例胃癌患者临床及随访资料.将手术切除标本制作组织芯片,以CD163作为M2型巨噬细胞标记,以Foxp3作为Tregs细胞标记,免疫组织化学染色后,分别以阳性细胞密度和阳性细胞计数来表示M2型巨噬细胞和Tregs细胞的表达.结果 肿瘤组织中M2巨噬细胞密度和Tregs阳性细胞计数中位数分别为7.48/HP和6.33/HP,高于癌旁组织的1.37/HP和2.92/HP(均P<0.01).胃癌组织内M2型巨噬细胞与调节性T细胞表达呈显著正相关(r=0.415.P<0.01).M2和Tregs均低表达的43例患者中位生存期为99.0个月,显著长于M2和Trege均高表达的45例患者(72.3个月,P<0.05).结论 联合检测M2型巨噬细胞和,Tregs表达这两种不同来源的抑制性免疫细胞可有效预测胃癌预后.
目的 探討胃癌組織中M2型巨噬細胞和調節性T細胞(Tregs)的錶達情況及其聯閤檢測對預後的預測價值.方法 收集1999年2月至2005年12月期間複旦大學附屬中山醫院行D2根治術併達到R0切除的135例胃癌患者臨床及隨訪資料.將手術切除標本製作組織芯片,以CD163作為M2型巨噬細胞標記,以Foxp3作為Tregs細胞標記,免疫組織化學染色後,分彆以暘性細胞密度和暘性細胞計數來錶示M2型巨噬細胞和Tregs細胞的錶達.結果 腫瘤組織中M2巨噬細胞密度和Tregs暘性細胞計數中位數分彆為7.48/HP和6.33/HP,高于癌徬組織的1.37/HP和2.92/HP(均P<0.01).胃癌組織內M2型巨噬細胞與調節性T細胞錶達呈顯著正相關(r=0.415.P<0.01).M2和Tregs均低錶達的43例患者中位生存期為99.0箇月,顯著長于M2和Trege均高錶達的45例患者(72.3箇月,P<0.05).結論 聯閤檢測M2型巨噬細胞和,Tregs錶達這兩種不同來源的抑製性免疫細胞可有效預測胃癌預後.
목적 탐토위암조직중M2형거서세포화조절성T세포(Tregs)적표체정황급기연합검측대예후적예측개치.방법 수집1999년2월지2005년12월기간복단대학부속중산의원행D2근치술병체도R0절제적135례위암환자림상급수방자료.장수술절제표본제작조직심편,이CD163작위M2형거서세포표기,이Foxp3작위Tregs세포표기,면역조직화학염색후,분별이양성세포밀도화양성세포계수래표시M2형거서세포화Tregs세포적표체.결과 종류조직중M2거서세포밀도화Tregs양성세포계수중위수분별위7.48/HP화6.33/HP,고우암방조직적1.37/HP화2.92/HP(균P<0.01).위암조직내M2형거서세포여조절성T세포표체정현저정상관(r=0.415.P<0.01).M2화Tregs균저표체적43례환자중위생존기위99.0개월,현저장우M2화Trege균고표체적45례환자(72.3개월,P<0.05).결론 연합검측M2형거서세포화,Tregs표체저량충불동래원적억제성면역세포가유효예측위암예후.
Objective To explore the prognostic value of M2 macrophages and regulatory T cells (Tregs) in gastric carcinoma. Methods Clinicopathological characteristics and follow up data of 135 patients with gastric carcinoma were collected. Patients included were those who underwent D2 radical resection (R0) at Zhongshan Hospital of Fudan University from February 1999 to December 2005. Tissue chips of gastric carcinoma specimen were stained using immunohistochemistry to determine the cells density and number of M2 (CD163 positive) and Tregs (Foxp3 positive). Results The median positive cells density of M2 macrophages and Tregs in tumor tissue were 7.48/HP and 6.33/HP, respectively, higher than that in adjacent tissues (1.37/HP and 2.92/HP,P<0.001). The density of M2 macrophages was positively correlated with that of Treg cells (r= 0.415, P<0.001) in tumor tissue. The median survival of patients with low expression of M2 and Tregs (n=43) was significantly longer than those with high expression of the 2 cells (n=45) (99.0 vs. 72.3 months,P<0.05). Conclusion Combined detection of M2 macrophages and Tregs may predict the prognosis of gastric carcinoma.
