CAJ | 학술논문

目的探讨急性白血病(AL)患者Wnt抑制因子1(WIF-1)基因启动子甲基化及β连环蛋白(β-catenin)的表达在白血病中的意义.方法采用甲基化特异性PCR检测山东大学附属千佛山医院2009年1月至2010年6月55例AL患者及对照组骨髓单个核细胞WIF-1基因启动子甲基化情况,流式细胞术检测AL患者及对照组骨髓单个核细胞β-catenin表达.结果 32.7％(18/55)的AL患者WIF-1基因启动子区域有异常甲基化,对照组末检测到WIF-1基因甲基化,两组差异有统计学意义(P＜0.05).甲基化组首次诱导治疗完全缓解率(38.9％,7/18)低于非甲基化组(81.1％,30/37),差异有统计学意义(P＜0.05).甲基化组与非甲基化组β-catenin的表达(17.5％±3.3％、15.4％±3.6％)均高于对照组(10.5％±1.5％,均P＜0.05)；甲基化组β-catenin的表达高于非甲基化组(P＜0.05).结论 WIF-1基因启动子甲基化及β-catenin过表达可能参与了AL患者Wnt/β-catenin途径的异常激活.
목적 탐토급성백혈병(AL)환자Wnt억제인자1(WIF-1)기인계동자갑기화급β련배단백(β-catenin)적표체재백혈병중적의의.방법 채용갑기화특이성PCR검측산동대학부속천불산의원2009년1월지2010년6월55례AL환자급대조조골수단개핵세포WIF-1기인계동자갑기화정황,류식세포술검측AL환자급대조조골수단개핵세포β-catenin표체.결과 32.7％(18/55)적AL환자WIF-1기인계동자구역유이상갑기화,대조조말검측도WIF-1기인갑기화,량조차이유통계학의의(P＜0.05).갑기화조수차유도치료완전완해솔(38.9％,7/18)저우비갑기화조(81.1％,30/37),차이유통계학의의(P＜0.05).갑기화조여비갑기화조β-catenin적표체(17.5％±3.3％、15.4％±3.6％)균고우대조조(10.5％±1.5％,균P＜0.05)；갑기화조β-catenin적표체고우비갑기화조(P＜0.05).결론 WIF-1기인계동자갑기화급β-catenin과표체가능삼여료AL환자Wnt/β-catenin도경적이상격활.
Objective To explore the significance of WIF-1 gene promoter methylation and the expression of β-catenin in acute leukemia(AL)patients.Methods The method of methylation specific polymerase chain reaction was employed to detect the status of WIF-1 gene methylation in 55 acute leukemia patientsand normal controls from January 2009 to June 2010 in our hospital.The expression of β-catenin was measured by flow cytometry.Results The methylation of WIF-1 gene promoter was found in 32.7％(18/55)AL patients.And the percentage was significantly higher than that of the controls(0).The patients with the methylation of WIF-1 gene had a lower complete remission rate(38.9％,7/18)for the first chemotherapy than those without(81.1％,30/37)(P ＜ 0.05).The expressions of β-catenin in methylation AL patients and those with non-methylation were 17.5％ ± 3.3％ and 15.4％ ± 3.6％ respectively.And they were significantly higher than the controls(10.5％ ± 1.5％,P ＜0.05).The expression of β-catenin was higher in positive methylation patients than those negative ones(P ＜ 0.05).Conclusion The methylation of WIF-1 gene promoter and β-catenin may be involved in the abnormal activation of Wnt/β-catenin signal in acute leukemia.