肿瘤研究与临床
腫瘤研究與臨床
종류연구여림상
CANCER RESEARCH AND CLINIC
2011年
3期
201-203
,共3页
庄建良%潘群雄%许荣誉%朱雄鹏%李纯团
莊建良%潘群雄%許榮譽%硃雄鵬%李純糰
장건량%반군웅%허영예%주웅붕%리순단
肺肿瘤%树突细胞%T淋巴细胞,细胞毒性%粒细胞巨噬细胞集落刺激因子
肺腫瘤%樹突細胞%T淋巴細胞,細胞毒性%粒細胞巨噬細胞集落刺激因子
폐종류%수돌세포%T림파세포,세포독성%립세포거서세포집락자격인자
Lung neoplasms%Dendritic cells%T-lymphocytes,cytotoxic%Granulocyte-macrophage colony-stimulating factor
目的 研究负载有肺癌细胞株A549可溶性抗原并携带外源粒细胞巨噬细胞集落刺激因子(GM-CSF)基因的树突状细胞(DC)在体外激活自身T淋巴细胞形成的细胞毒T淋巴细胞(CTL)对A549细胞的杀伤作用.方法 用肺癌细胞株A549可溶性抗原致敏DC,再用含有GM-CSF外源基因的腺病毒感染DC,将所得的DC与T细胞混合培养以形成对A549细胞有特异杀伤作用的CTL.细胞分为未处理(N-DC)组、加抗原未感染病毒(A-DC)组和加抗原感染病毒(G-A-DC)组.通过测定乳酸脱氢酶(LDH)计算CTL对A549细胞的杀伤率.结果 当CTL与A549细胞,即效应细胞与靶细胞比值(E/T)为5∶1时,N-DC组的杀伤率为(1.9±0.7)%,A-DC组为(21.3±2.6)%,G-A-DC组为(34.5±4.9)%;当E/T为10∶1时,N-DC组的杀伤率为(4.8±0.8)%,U-DC组为(35.4±3.6)%,G-A-DC组为(51.3±2.9)%;E/T为20∶1时,N-DC组的杀伤率为(5.3±0.2)%,A-DC组为(40.5±7.7)%,G-A-DC组为(72.5±4.7)%.3组之间的杀伤率差异有统计学意义(n=2,F=356,P＜0.05),通过两两比较,得出G-A-DC组的杀伤率高于其他两组(P＜0.001),且A-DC组高于对照组(P＜0.001).结论 以肺癌细胞株A549可溶性抗原致敏的DC可诱导出对A549具有特异杀伤作用的CTL,当所致敏的DC通过腺病毒感染而带有外源基因GM-CSF时,所诱导的细胞毒杀伤反应进一步增强.
目的 研究負載有肺癌細胞株A549可溶性抗原併攜帶外源粒細胞巨噬細胞集落刺激因子(GM-CSF)基因的樹突狀細胞(DC)在體外激活自身T淋巴細胞形成的細胞毒T淋巴細胞(CTL)對A549細胞的殺傷作用.方法 用肺癌細胞株A549可溶性抗原緻敏DC,再用含有GM-CSF外源基因的腺病毒感染DC,將所得的DC與T細胞混閤培養以形成對A549細胞有特異殺傷作用的CTL.細胞分為未處理(N-DC)組、加抗原未感染病毒(A-DC)組和加抗原感染病毒(G-A-DC)組.通過測定乳痠脫氫酶(LDH)計算CTL對A549細胞的殺傷率.結果 噹CTL與A549細胞,即效應細胞與靶細胞比值(E/T)為5∶1時,N-DC組的殺傷率為(1.9±0.7)%,A-DC組為(21.3±2.6)%,G-A-DC組為(34.5±4.9)%;噹E/T為10∶1時,N-DC組的殺傷率為(4.8±0.8)%,U-DC組為(35.4±3.6)%,G-A-DC組為(51.3±2.9)%;E/T為20∶1時,N-DC組的殺傷率為(5.3±0.2)%,A-DC組為(40.5±7.7)%,G-A-DC組為(72.5±4.7)%.3組之間的殺傷率差異有統計學意義(n=2,F=356,P＜0.05),通過兩兩比較,得齣G-A-DC組的殺傷率高于其他兩組(P＜0.001),且A-DC組高于對照組(P＜0.001).結論 以肺癌細胞株A549可溶性抗原緻敏的DC可誘導齣對A549具有特異殺傷作用的CTL,噹所緻敏的DC通過腺病毒感染而帶有外源基因GM-CSF時,所誘導的細胞毒殺傷反應進一步增彊.
목적 연구부재유폐암세포주A549가용성항원병휴대외원립세포거서세포집락자격인자(GM-CSF)기인적수돌상세포(DC)재체외격활자신T림파세포형성적세포독T림파세포(CTL)대A549세포적살상작용.방법 용폐암세포주A549가용성항원치민DC,재용함유GM-CSF외원기인적선병독감염DC,장소득적DC여T세포혼합배양이형성대A549세포유특이살상작용적CTL.세포분위미처리(N-DC)조、가항원미감염병독(A-DC)조화가항원감염병독(G-A-DC)조.통과측정유산탈경매(LDH)계산CTL대A549세포적살상솔.결과 당CTL여A549세포,즉효응세포여파세포비치(E/T)위5∶1시,N-DC조적살상솔위(1.9±0.7)%,A-DC조위(21.3±2.6)%,G-A-DC조위(34.5±4.9)%;당E/T위10∶1시,N-DC조적살상솔위(4.8±0.8)%,U-DC조위(35.4±3.6)%,G-A-DC조위(51.3±2.9)%;E/T위20∶1시,N-DC조적살상솔위(5.3±0.2)%,A-DC조위(40.5±7.7)%,G-A-DC조위(72.5±4.7)%.3조지간적살상솔차이유통계학의의(n=2,F=356,P＜0.05),통과량량비교,득출G-A-DC조적살상솔고우기타량조(P＜0.001),차A-DC조고우대조조(P＜0.001).결론 이폐암세포주A549가용성항원치민적DC가유도출대A549구유특이살상작용적CTL,당소치민적DC통과선병독감염이대유외원기인GM-CSF시,소유도적세포독살상반응진일보증강.
Objective The goal of the study was to assess the lethal effect of cytotoxic lymphocyte against A549 cells induced by dendritic cells (DC) pulsed with A549 lysate and transfected with GM-CSF recombinant adenovirus. Methods The cytotoxic lymphocyte against A549 cells were induced by culturing with DCs, which pulsed with A549 antigens and transfected with GM-CSF recombinant adenovirus. The of effector/target ratio, the killing rates of N-DC group, A-DC group and G-A-DC group were (1.9±0.7) %,effector/target ratio, the killing rates of N-DC group, A-DC group and G-A-DC group were (5.3±0.2) %, (40.5±7.7) % and (72.5±4.7) %, respectively. We found that the killing rate of G-A-DC group was the highest by statistics. Conclusion The cytotoxic lymphocyte against A549 cells can be induced by DCs pulsed with A549 lysate ,and the lethal effect of CTLs can be enhanced when DCs were infected with GM-CSF recombinant adenovirus.
