CAJ | 학술논문

用RAPD分子标记技术及数量遗传分析手段对小麦异交群体趋异选择4代得到的各子群体的遗传关系进行了分析，并对两种结果进行了相关分析。采用11个引物扩增出134个位点，RAPD分析结果表明，群体具有丰富的遗传变异，整个群体的多态位点百分率达0.8134，杂合度达0.3006；子群体间遗传分化较小，相对基因分化系数Gst为0.2310，固定指数平均0.2120，标准遗传距离为0.1044±0.048，表明遗传变异多数来自群体之内。而数量遗传分析发现，选择性状进展都与预期方向相符，主成分遗传距离大部分达显著水平，说明选择使群体发生了相当程度的分化。对两种遗传距离进行聚类分析表明，二种信息间关系各类群成员多不一致。其原因可能在于：数量性状是人工选择的目标，RAPD是基因组随机序列的扩增，二者之间的相关与引物选择有关。
용RAPD분자표기기술급수량유전분석수단대소맥이교군체추이선택4대득도적각자군체적유전관계진행료분석，병대량충결과진행료상관분석。채용11개인물확증출134개위점，RAPD분석결과표명，군체구유봉부적유전변이，정개군체적다태위점백분솔체0.8134，잡합도체0.3006；자군체간유전분화교소，상대기인분화계수Gst위0.2310，고정지수평균0.2120，표준유전거리위0.1044±0.048，표명유전변이다수래자군체지내。이수량유전분석발현，선택성상진전도여예기방향상부，주성분유전거리대부분체현저수평，설명선택사군체발생료상당정도적분화。대량충유전거리진행취류분석표명，이충신식간관계각류군성원다불일치。기원인가능재우：수량성상시인공선택적목표 ，RAPD시기인조수궤서렬적확증，이자지간적상관여인물선택유관 。
The genetic relations of some subpopulations in the fourth generation of selection in outbreeding population of winter wheat were examined by RAPD techniques and quantitative analysis. The correlations of the two results were examined.For RAPD analysis, 11 primers were adopted, and 134 sites were amplified. The results showed that abundant genetic variations existed in the populations. In the whole population, the percentage of polymorphic sites (P) is 0.8134, and the averaged heterozygosity is 0.3006. However,genetic differentiation among subpopulations is small. The relative gene differentiation (Gst) is 0.2310,fixed index averages 0.2120, and the standard genetic distance between subpopulations is 0.1044±0.048.All above shows that most of the genetic variations existed within populations.The cluster results from RAPD analysis and principal component distances showed that similarity relations were only found in small part of the subpopulations. The reason for the results may be that the genes concerned for quantitative traits are selected directly，while the genes involved in RAPD analysis are only random samples of the whole genomes.