重庆医科大学学报
重慶醫科大學學報
중경의과대학학보
UNIVERSITATIS SCIENTIAE MEDICINAE CHONGQING
2001年
1期
51-52,55
,共3页
易岂建%杨锡强%李成荣%张远维%王莉佳
易豈建%楊錫彊%李成榮%張遠維%王莉佳
역기건%양석강%리성영%장원유%왕리가
川崎病%细胞凋亡%p53基因%白细胞介素
川崎病%細胞凋亡%p53基因%白細胞介素
천기병%세포조망%p53기인%백세포개소
目的：进一步探讨川崎病(KD)急性期患者外周血淋巴细胞凋亡延迟的机理。方法：采用斑点杂交(Dot-blot)检测淋巴细胞p53基因mRNA表达水平；流式细胞仪(FCM)检测p53蛋白质表达阳性细胞百分率。结果：KD患者外周血淋巴细胞p53基因mRNA和p53蛋白质表达水平降低，与正常儿童比较差异显著(P＜0.005)；当给予静脉注射免疫球蛋白(IVIG)治疗后或加入抗IL-6单抗培养时，外周血淋巴细胞p53基因mRNA和P53蛋白质表达水平提高。结论：KD急性期患者外周血淋巴细胞p53基因表达水平降低，其原因可能与本病患者异常升高的IL-6有关。p53基因具有促进细胞凋亡的作用，KD患者外周血淋巴细胞凋亡延迟可能与高浓度IL-6抑制p53基因的表达有关。
目的：進一步探討川崎病(KD)急性期患者外週血淋巴細胞凋亡延遲的機理。方法：採用斑點雜交(Dot-blot)檢測淋巴細胞p53基因mRNA錶達水平；流式細胞儀(FCM)檢測p53蛋白質錶達暘性細胞百分率。結果：KD患者外週血淋巴細胞p53基因mRNA和p53蛋白質錶達水平降低，與正常兒童比較差異顯著(P＜0.005)；噹給予靜脈註射免疫毬蛋白(IVIG)治療後或加入抗IL-6單抗培養時，外週血淋巴細胞p53基因mRNA和P53蛋白質錶達水平提高。結論：KD急性期患者外週血淋巴細胞p53基因錶達水平降低，其原因可能與本病患者異常升高的IL-6有關。p53基因具有促進細胞凋亡的作用，KD患者外週血淋巴細胞凋亡延遲可能與高濃度IL-6抑製p53基因的錶達有關。
목적：진일보탐토천기병(KD)급성기환자외주혈림파세포조망연지적궤리。방법：채용반점잡교(Dot-blot)검측림파세포p53기인mRNA표체수평；류식세포의(FCM)검측p53단백질표체양성세포백분솔。결과：KD환자외주혈림파세포p53기인mRNA화p53단백질표체수평강저，여정상인동비교차이현저(P＜0.005)；당급여정맥주사면역구단백(IVIG)치료후혹가입항IL-6단항배양시，외주혈림파세포p53기인mRNA화P53단백질표체수평제고。결론：KD급성기환자외주혈림파세포p53기인표체수평강저，기원인가능여본병환자이상승고적IL-6유관。p53기인구유촉진세포조망적작용，KD환자외주혈림파세포조망연지가능여고농도IL-6억제p53기인적표체유관。
Objective: To further explore the mechanism of inhibited apoptosis of peripheral blood mononuclear cell (PBMC) in acute Kawasaki disease(KD). Methods: The expression level of p53 gene mRNA was determined by dot-blot; p53 protein positive cell percentage was detected by flow cytometry (FCM). Results:The expression of p53 gene mRNA and p53 protein in acute KD patients were decreased(P＜0.001), but increased after treating with intravenous immunoglobulin(IVIG) in vivo or adding anti-IL-6 monoantibody(mAb)into PBMC culture in vitro. Conclusion. The decreased expression of p53 gene mRNA and p53 protein may be associated with the high concentration of IL-6 in KD patients, p53 gene expression could induce lymphocyte apoptosis. Thus, the expression of p53 gene inhibited by the increased IL-6 production might be related to delaying or depressing apoptosis of PBMC in KD.
