CAJ | 학술논문

本文建立了一种快速、可靠的急性分离成年大鼠海马神经细胞的方法.此法可将实验大鼠的年龄提高到500 d以上,体重300 g以上;不损伤神经细胞膜的电学特性;形态上有差异的细胞易于分辨.用膜片钳技术的单通道和全细胞模式证实,在本实验条件下,约95%左右的健康细胞均能形成高阻抗封接,并成功地记录了电压依赖性钾、钠、钙通道,外向整流氯通道和大电导的钙激活钾通道电流.
본문건립료일충쾌속、가고적급성분리성년대서해마신경세포적방법.차법가장실험대서적년령제고도500 d이상,체중300 g이상;불손상신경세포막적전학특성;형태상유차이적세포역우분변.용막편겸기술적단통도화전세포모식증실,재본실험조건하,약95%좌우적건강세포균능형성고조항봉접,병성공지기록료전압의뢰성갑、납、개통도,외향정류록통도화대전도적개격활갑통도전류.
An improved method is described for fast and reliable isolation of neurons from hippocampus of adult rats by acombination of mechanical and enzymatic means. The procedure allows the isolation of neurons from 500～600-d-old rats (over300 g), preserving the proximal dendritic structure without impairing the electrical characteristics of the cells. Morphologicallydistinct neurons can be recognized. Using cell-attached, inside-out and whole-cell configurations of patch clamp technique, it wasshown that the enzymatically isolated neurons in hippocampus from rats weighing more than 300 g exhibited voltage-gatedcalcium, sodium and potassium currents, outwardly rectifying chloride channel and large conductance Ca2+-activated potassiumchannel currents. Approximately, 95% of healthy cells allowed the formation of giga-ohm seals.