CAJ | 학술논문

目的:研究经鼻给小剂量重组人促红细胞生成素(r-HuEPO)对大鼠癫痫发作的影响.方法:大鼠经鼻或腹腔给r-HuEPO后,观察对戊四唑(PTZ)诱导癫痫发作潜伏时间、级别、大发作持续时间、次数的影响.另外,大鼠经鼻给r-HuEPO后,观察对最大电休克(MES)诱导的惊厥发作级别,前肢或后肢强直性伸展时间的影响;对杏仁核电点燃后阈刺激诱导的行为等级,大发作持续时间及后放电的影响.结果:经鼻给予小剂量r-HuEPO,可明显延长PTZ诱导的癫痫发作潜伏时间,降低癫痫发作级别、大发作持续时间和发作次数,其中2.4 IU剂量组作用最为明显.而腹腔给r-HuEPO(3 000、4 000 IU/kg),仅可减少大发作次数和缩短大发作持续时间.经鼻给2.4 IU的r-HuEPO,可显著降低MES诱导的大鼠惊厥发作级别,缩短前肢和后肢强直性伸展时间.另外,经鼻给2.4 IU r-HuEPO,明显降低杏仁核电点燃后阈刺激诱导的癫痫发作级别,缩短大发作时间和后放电时间.结论:经鼻给小剂量的r-HuEPO有明显的抗癫痫作用.
목적:연구경비급소제량중조인촉홍세포생성소(r-HuEPO)대대서전간발작적영향.방법:대서경비혹복강급r-HuEPO후,관찰대무사서(PTZ)유도전간발작잠복시간、급별、대발작지속시간、차수적영향.령외,대서경비급r-HuEPO후,관찰대최대전휴극(MES)유도적량궐발작급별,전지혹후지강직성신전시간적영향;대행인핵전점연후역자격유도적행위등급,대발작지속시간급후방전적영향.결과:경비급여소제량r-HuEPO,가명현연장PTZ유도적전간발작잠복시간,강저전간발작급별、대발작지속시간화발작차수,기중2.4 IU제량조작용최위명현.이복강급r-HuEPO(3 000、4 000 IU/kg),부가감소대발작차수화축단대발작지속시간.경비급2.4 IU적r-HuEPO,가현저강저MES유도적대서량궐발작급별,축단전지화후지강직성신전시간.령외,경비급2.4 IU r-HuEPO,명현강저행인핵전점연후역자격유도적전간발작급별,축단대발작시간화후방전시간.결론:경비급소제량적r-HuEPO유명현적항전간작용.
Objective: To investigate the effect of intranasal administration of low dosage recombinant human erythropoietin (r-HuEPO) on seizure in rats. Methods: After intranasal or intraperitoneal administration of r-HuEPO,the behavioral and electroencephalographic changes were observed in pentylenetetrazol (PTZ) and maximal electroshock (MES) induced seizure or electrical amygdaloid-kindled seizure of rats. Results: Intranasal administration of low dosage r-HuEPO increased the seizure latency,and decreased the seizure grade and duration,and the number of convulsive episodes in PTZ induced seizure,with the most potential dosage of 2.4 IU.Intraperitoneal administration of r-HuEPO (3 000,4 000 IU/kg) only decreased the seizure duration and number of convulsive episodes.The seizure grade,forelimb or hindlimb extension duration were decreased in MES-induced seizure by intranasal administration of 2 4 IU r-HuEPO.In addition,intranasal administration of 2.4 IU r-HuEPO decreased the seizure grade,generalized seizure duration and afterdischarge in electrical amygdaloid-kindled rats stimulated with generalized seizure threshold. Conclusion: Intranasal administration of low dosage r-HuEPO can inhibit the seizure in rats.