CAJ | 학술논문

[目的]探讨桫椤科植物DNA提取的最优方法.[方法]采用经过改良的SDS法和CTAB法对3种桫椤科植物进行基因组DNA提取,用紫外分光光度计测定其在波长260和280 nm处的吸光值,并根据A_(260 nm)/A_(280 nm)的比值分析其DNA的纯度.将提取的基因组DNA进行叶绿体trnL-trnF非编码区序列扩增和ISSR 分析.[结果]改进的CTAB法所提出的DNA较纯净,所获得DNA样品的A_(260 nm)/A_(280 nm)比值在1.750～2.050范围内,琼脂糖凝胶电泳图谱显示条带清晰.[结论]改良的CTAB法更适合桫椤科植物DNA的提取.
[목적]탐토사라과식물DNA제취적최우방법.[방법]채용경과개량적SDS법화CTAB법대3충사라과식물진행기인조DNA제취,용자외분광광도계측정기재파장260화280 nm처적흡광치,병근거A_(260 nm)/A_(280 nm)적비치분석기DNA적순도.장제취적기인조DNA진행협록체trnL-trnF비편마구서렬확증화ISSR 분석.[결과]개진적CTAB법소제출적DNA교순정,소획득DNA양품적A_(260 nm)/A_(280 nm)비치재1.750～2.050범위내,경지당응효전영도보현시조대청석.[결론]개량적CTAB법경괄합사라과식물DNA적제취.
[Objective]To explore an efficient method for extracting genomic DNA from Cyatheaceae plants.[Method]The improved sodium dodecy sulphate (SDS) and cety triethl ammonium bromide (CTAB) methods were used for the genomic DNA extraction of three kinds of Cyatheaceae plants.The absorption values were deter mined by ultraviolet spectrophotometry at A_(260 nm) and A_(280 nm) ,and the DNA purities were analyzed by A_(260 nm)/A_(280 nm) ratio.The amplification of chloroplast trnL-trnF non-coding region and ISSR analysis of genomic DNA were then conducted.[Result]The results showed that the improved CTAB method of extracting genomic DNA from Cyatheaceae plants was better than the improved SDS method,and the DNA purity of the former was higher than that of the latter.The A_(260 nm)/A_(280 nm) ratio of the extracted DNA with the improved CTAB method was in the range from 1.750 to 2.050,and distinct bands were found in the agarose gel electrophoresis map of ISSR.[Conclusion]The improved CTAB method is more suitable for the DNA extraction of Cyatheaceae plant.