CAJ | 학술논문

目的: 研究角膜移植后角膜新生淋巴管与新生血管间关联.方法:人角膜取自行二次角膜移植的19名患者.5'核苷酸酶-碱性磷酸酶(5'-nase-Alkaline phosphatase ,5'-NA-ALP)双重酶组织化学染色及淋巴内皮细胞受体(lymphatic vessel endothelial receptor, LYVE-1)、内皮细胞黏附因子-1(platelet endothelial cell adhesion modecule-1, PECAM-1)双重免疫组化法标记角膜中的新生血管和淋巴管,并进行淋巴管计数(lymphatic vessels counting, LVC)和血管计数(blood vessels counting, BVC),比较BVC与LVC之间的关联.结果: 角膜中存在角膜新生血管12例(63%),存在角膜新生淋巴管5例(26%).角膜新生淋巴管仅出现在血管化角膜中.角膜移植后BVC 与LVC间呈显著性正相关(r= 0.725;P<0.01).结论:人角膜移植后角膜新生淋巴管与新生血管之间存在密切关联.
목적: 연구각막이식후각막신생림파관여신생혈관간관련.방법:인각막취자행이차각막이식적19명환자.5'핵감산매-감성린산매(5'-nase-Alkaline phosphatase ,5'-NA-ALP)쌍중매조직화학염색급림파내피세포수체(lymphatic vessel endothelial receptor, LYVE-1)、내피세포점부인자-1(platelet endothelial cell adhesion modecule-1, PECAM-1)쌍중면역조화법표기각막중적신생혈관화림파관,병진행림파관계수(lymphatic vessels counting, LVC)화혈관계수(blood vessels counting, BVC),비교BVC여LVC지간적관련.결과: 각막중존재각막신생혈관12례(63%),존재각막신생림파관5례(26%).각막신생림파관부출현재혈관화각막중.각막이식후BVC 여LVC간정현저성정상관(r= 0.725;P<0.01).결론:인각막이식후각막신생림파관여신생혈관지간존재밀절관련.
AIM: To examine the relationship between corneal lymphangiogenesis and hemangiogenesis after keratoplasty.METHODS: Nineteen human corneas were obtained from 19 patients undergoing a second corneal transplantation in Zhongshan Ophthalmic Center in 2005. Blood and lymphatic vessels in human transplanted corneas were identified by lymphatic vessel endothelial receptor(LYVE-1) and platelet endothelial cell adhesion modecule-1(PECAM-1) immunohistochemistry, and double enzyme-histochemistry; then the association of corneal blood vessel counting (BVC) with lymphatic vessel counting (LVC) was examined.RESULTS: Corneal hemangiogenesis was present in 12 cases (63%), and lymphangiogenesis occurred in 5 cases (26%) of human transplanted corneas. In addition, corneal lymphangiogenesis was only present in vascularized corneas. LVC was strongly and positively correlated with BVC(r=0.725;P<0.01).CONCLUSION: Corneal lymphangiogenesis develops after keratoplasty, and strongly associates with hemangiogenesis.