CAJ | 학술논문

为了解栽培种甘薯(徐薯18,lpomoea batatas cv.Xushu No.18)的染色体结构,文章利用 45S rDNA荧光原位杂交、自身基因组荧光原位杂交和银染技术对栽培种甘薯进行分子细胞遗传学研究.银染结果显示,徐薯18问期核有6对、8对和9对银染点;45S rDNA荧光原位杂交结果显示,徐薯18染色体上有8对或9对强弱不一的45S rDNA信号;自身基因组荧光原位杂交结果表明,所有染色体的全长分布强烈而密集的杂交信号,着丝粒区、近着丝粒区和端粒区有增强的信号带.
위료해재배충감서(서서18,lpomoea batatas cv.Xushu No.18)적염색체결구,문장이용 45S rDNA형광원위잡교、자신기인조형광원위잡교화은염기술대재배충감서진행분자세포유전학연구.은염결과현시,서서18문기핵유6대、8대화9대은염점;45S rDNA형광원위잡교결과현시,서서18염색체상유8대혹9대강약불일적45S rDNA신호;자신기인조형광원위잡교결과표명,소유염색체적전장분포강렬이밀집적잡교신호,착사립구、근착사립구화단립구유증강적신호대.
In order to understand the chromosome structure of sweet potato(Ipomoea batatas cv.Xushu 18),molecular cytogenetic analyses were carried out on I. batatas.by using 45S rDNA fluorescence in situ hybridization(45S rDNA-FISH),self genomic in situ hybridization(self-GISH),and silver staining techniques.Twelve,sixteen,and eighteen regions were silver stained in the interphase nucleus of I.batatas.The results of FISH analysis demonstrated 16 or 18 signals with different intensity on chromosomes of I.batatas.Self-GISH analysis showed that the intensive signals on I.batatas mitotic chromosomes were distributed along the chromosomes.However,the signals located in centromeric.subcentromeric,and telomeric regions were stronger and denser than those in other regions.