CAJ | 학술논문

目的:研究了从福建连江海域分离得到的一株野生底栖硅藻(Nitzschia.constricta)的生长和生理特性.方法:测定了在不同氮磷浓度条件下比增长速率、色素,以及不同时期底栖硅藻蛋白和胞外多糖的积累情况.结果:当硝酸氮浓度为900mg/l时,获得最大比增长速率0.21和叶绿素a含量4.31mg/l、类胡萝卜素含量3.44mg/l、胞外多糖产率90.57μg/ml.当氮浓度为75mg/l时,在第10d得到最大蛋白产率28.90μg/ml.当磷浓度为4.4mg/l时,可得到最大比增长速率0.13、叶绿素a含量3.42mg/l、类胡萝卜素2.82mg/l、蛋白含量23.16μg/ml,无磷培养基中第5d的胞外多糖产率最高13.51μg/ml.结论:氮磷营养盐浓度的增加促进了此种底栖硅藻的生长,但不一定会促进蛋白及胞外多糖的产生.
목적:연구료종복건련강해역분리득도적일주야생저서규조(Nitzschia.constricta)적생장화생리특성.방법:측정료재불동담린농도조건하비증장속솔、색소,이급불동시기저서규조단백화포외다당적적루정황.결과:당초산담농도위900mg/l시,획득최대비증장속솔0.21화협록소a함량4.31mg/l、류호라복소함량3.44mg/l、포외다당산솔90.57μg/ml.당담농도위75mg/l시,재제10d득도최대단백산솔28.90μg/ml.당린농도위4.4mg/l시,가득도최대비증장속솔0.13、협록소a함량3.42mg/l、류호라복소2.82mg/l、단백함량23.16μg/ml,무린배양기중제5d적포외다당산솔최고13.51μg/ml.결론:담린영양염농도적증가촉진료차충저서규조적생장,단불일정회촉진단백급포외다당적산생.
Objective:The growth and physiological parameters of the benthic diatom(Nitzschia.constricta)isolated from Lianjiang maritime space in Fujian were studied.Method: The pigments,growth rate, protein and exopolysaecharide yield were evaluated at several nitrogen and phosphorus levels on different days.Result: When the nitrate concentration is 900mg/l, the highest growth rate 0.21, chlorophylla 4.31mg/l and carotenoid 3.44mg/l,and the production of exopolysaeeharide 90.57μg/ml were obtained.While the most protein content 28.90μg/ml was obtained on the 10th day under the nitrogen concentration 75mg/l.When the phosphorus concentration is 4.4mg/ml , the highest growth rate 0.13, chlorophylla 3.42mg/l, carotenoid 2.82mg/l and the very protein content 23.16μg/ml can be acquired.However the most exopolysaccharide yield was attained to 13.51μg/ml at non -phosphorus media on the 5th day.Conclusion: The in-creasing nitrogen and phosphorus concentration can elevate the specific growth rate and pigments of the benthic diatom(Nitzschia, constric-ta) ,but will not be sure to promote the protein and exopolysaccharide yield.