CAJ | 학술논문

利用AFLP分子标记技术分析了25份甘蓝型油菜品种的遗传多样性.9对AFLP引物扩增出193条带,其中,73条呈多态性,多态性比率为38％.每对引物检测出的平均等位基因数为3.56,有效等位基因数为1.41.基因多样性、香农指数和遗传差异分别为0.25、0.62和0.39.在遗传相似系数0.66处,所有的甘蓝型油菜可分为3个类群,遗传相似系数表明,橄榄型油菜表现出丰富的遗传多样性.UPGMA聚类分析表明,品种间的亲缘关系与种质性状或来源关系不明显.
이용AFLP분자표기기술분석료25빈감람형유채품충적유전다양성.9대AFLP인물확증출193조대,기중,73조정다태성,다태성비솔위38％.매대인물검측출적평균등위기인수위3.56,유효등위기인수위1.41.기인다양성、향농지수화유전차이분별위0.25、0.62화0.39.재유전상사계수0.66처,소유적감람형유채가분위3개류군,유전상사계수표명,감람형유채표현출봉부적유전다양성.UPGMA취류분석표명,품충간적친연관계여충질성상혹래원관계불명현.
AFLP markers were applied to assess genetic diversity and relationships among 25 B.napus cultivars.Nine AFLP primer combinations generated 193 fragments,of which 73 were polymorphic.The polymorphic rate was 38％.The average number of observed alleles per locus was 3.56,while the mean number of effective alleles was 1.41.Gene diversity,Shannon' s information index,and genetic differentiation were 0.25,0.62,and 0.39,respectively.At a genetic similarity coefficient of 0.66,all B.napus cultivars could be divided into three groups.A wide range of similarity coeffcient value suggested that the investigated B.napus germplasm collection represented a genetically diverse population.UPGMA cluster analysis revealed that no apparent clustering by traits or original locations.