国际呼吸杂志
國際呼吸雜誌
국제호흡잡지
INTERNATIONAL JOURNAL OF RESPIRATION
2011年
21期
1625-1628
,共4页
肺腺癌%Notch1%实时荧光定量PCR
肺腺癌%Notch1%實時熒光定量PCR
폐선암%Notch1%실시형광정량PCR
Lung adenocarcinoma%Notch1%Real-time fluorescence quantitative PCR
目的 研究Notch1蛋白在人肺腺癌组织中的表达及意义,探讨Notch1受体在人肺腺癌发生中的作用.方法 前期研究初步筛选出Notch1蛋白在肺腺癌组织中有强表达,本研究收集我院及新华医院2009年1月至2010年5月胸外科肺癌手术并经术后病理切片明确为肺腺癌的标本以及正常肺组织标本,以实时荧光定量PCR及Western blot方法分别检测肺癌组织中Notch1 mRNA及蛋白的表达水平,进一步探讨Notch1在肺腺癌中所发挥的作用.结果 实时荧光定量PCR分析显示,Notch1mRNA的表达在肺腺癌组织及正常肺组织中分别为1.99±1.40和1.50±1.03,组间比较差异有统计学意义(P ＜0.05)；Western blot分析显示,Notch1蛋白在肺腺癌组织及正常肺组织中分别为0.64±0.25和0.14±0.07,组间比较差异有统计学意义(P＜0.01).结论 Notch1信号在肺腺癌的发病机制中可能起促进作用.
目的 研究Notch1蛋白在人肺腺癌組織中的錶達及意義,探討Notch1受體在人肺腺癌髮生中的作用.方法 前期研究初步篩選齣Notch1蛋白在肺腺癌組織中有彊錶達,本研究收集我院及新華醫院2009年1月至2010年5月胸外科肺癌手術併經術後病理切片明確為肺腺癌的標本以及正常肺組織標本,以實時熒光定量PCR及Western blot方法分彆檢測肺癌組織中Notch1 mRNA及蛋白的錶達水平,進一步探討Notch1在肺腺癌中所髮揮的作用.結果 實時熒光定量PCR分析顯示,Notch1mRNA的錶達在肺腺癌組織及正常肺組織中分彆為1.99±1.40和1.50±1.03,組間比較差異有統計學意義(P ＜0.05)；Western blot分析顯示,Notch1蛋白在肺腺癌組織及正常肺組織中分彆為0.64±0.25和0.14±0.07,組間比較差異有統計學意義(P＜0.01).結論 Notch1信號在肺腺癌的髮病機製中可能起促進作用.
목적 연구Notch1단백재인폐선암조직중적표체급의의,탐토Notch1수체재인폐선암발생중적작용.방법 전기연구초보사선출Notch1단백재폐선암조직중유강표체,본연구수집아원급신화의원2009년1월지2010년5월흉외과폐암수술병경술후병리절편명학위폐선암적표본이급정상폐조직표본,이실시형광정량PCR급Western blot방법분별검측폐암조직중Notch1 mRNA급단백적표체수평,진일보탐토Notch1재폐선암중소발휘적작용.결과 실시형광정량PCR분석현시,Notch1mRNA적표체재폐선암조직급정상폐조직중분별위1.99±1.40화1.50±1.03,조간비교차이유통계학의의(P ＜0.05)；Western blot분석현시,Notch1단백재폐선암조직급정상폐조직중분별위0.64±0.25화0.14±0.07,조간비교차이유통계학의의(P＜0.01).결론 Notch1신호재폐선암적발병궤제중가능기촉진작용.
Objective To investigate the expression of Notch1 and its role in the pathogenesis in adult adenocarcinoma of lung.Methods The high expression of Notch1 was detected in lung adenocarcinoma in our previous study.In this investigation,operation specimens of adenocarcinoma of lung that were proved by pathological sections were collected.Notch1 mRNA and protein in lung adenocarcinoma were measured with real-time fluorescence quantitative PCR and Western blot respectively to investigate the role of Notchl in adenocarcinoma of lung.Results It was shown by real-time fluorescence quantitative PCR that the expression of Notch1 mRNA in lung adenocarcinoma group (1.99+1.40) was higher than that in normal lung group (1.50+1.03) ( P ＜0.05).The expression of Notch1 protein in lung adenocarcinoma group (0.64+0.25) was significantly higher than that in normal lung group ( 0.14 + 0.07 ) ( P ＜ 0.01 ) by Western blot.Conclusions Notch1 signal may play a stimulative role in the pathogenesis of lung adenocarcinoma.
