CAJ | 학술논문

目的通过采用高效液相色谱法来测定根痛平滴丸中芍药苷和葛根素的含量.方法应用AgelaTMC18色谱柱,水-甲醇-乙腈为流动相,流速为0.96 ml/min,柱温为30℃,250 nm的检测波长,测定根痛平滴丸中芍药苷和葛根素的含量并作相关分析.结果本品中每丸含芍药苷不小于0.80 mg,葛根素不小于0.28 mg,芍药苷于59～1150 mg/L,葛根素于9.0～180 mg/L的范围内线性关系好.结论采用高效液相色谱法测定药物成分含量,操作简单、结果精确,回收率良好和重现性满意,用于根痛平滴丸质量控制可获得良好效果.
목적 통과채용고효액상색보법래측정근통평적환중작약감화갈근소적함량.방법 응용AgelaTMC18색보주,수-갑순-을정위류동상,류속위0.96 ml/min,주온위30℃,250 nm적검측파장,측정근통평적환중작약감화갈근소적함량병작상관분석.결과 본품중매환함작약감불소우0.80 mg,갈근소불소우0.28 mg,작약감우59～1150 mg/L,갈근소우9.0～180 mg/L적범위내선성관계호.결론 채용고효액상색보법측정약물성분함량,조작간단、결과정학,회수솔량호화중현성만의,용우근통평적환질량공제가획득량호효과.
Objective To detect the contents of paeoniflorin and pueratin in root pain ping pills by using high performance liquid chromatography.Methods Agela TMC 18 column was used and water methanol acetonitrile was used as mobile phase,with a flow rate of 0.96 ml / min,column temperature of 30°C,and detection wavelength of 250 nm,to determine the contents of paeoniflorin and puerarin in root pain ping pills; and the related analysis was conducted.Results Paeoniflorin was not less than 0.80 mg and puerarin was not less than 0.28 mg in each pill.Paeoniflorin in 59～ 1150 mg / L and puerarin in 9～180 mg/ L had a good linear relation.Conclusions High performance liquid chromatography for detecting drug content is simple to operate and accurate and has good reproducibility and a satisfied recovery rate.It has a good effect for quality control of root pain ping pillat.