中华胰腺病杂志
中華胰腺病雜誌
중화이선병잡지
CHINESE JOURNAL OF PANCREATOLOGY
2011年
1期
34-36
,共3页
石毅%孙跃明%白剑锋%陆文熊%傅赞%奚春华%赵翰林%苗毅
石毅%孫躍明%白劍鋒%陸文熊%傅讚%奚春華%趙翰林%苗毅
석의%손약명%백검봉%륙문웅%부찬%해춘화%조한림%묘의
胰腺肿瘤%STAT5转录因子%生长激素%信号转导
胰腺腫瘤%STAT5轉錄因子%生長激素%信號轉導
이선종류%STAT5전록인자%생장격소%신호전도
Pancreatic neoplasms%STAT5 transcription factor%Growth hormone%Signal transducing
目的 检测磷酸化转录激活因子5(pSTAT5)在7株胰腺癌细胞株中的表达,观察生长激素(GH)处理SW1990细胞及其移植瘤后pSTAT5表达的变化,探讨GH的分子作用机制.方法 体外培养人胰腺癌细胞株SW1990、Cap-1、Colo、Mia、Aspc、P3、PANC1,Western blotting检测各株细胞的pSTAT5表达;收集指数生长期的SW1990细胞接种于BALB/C裸鼠,成瘤后随机分为GH组(瘤内注入GH 4 mg·kg-1·d-1,连续2周)和对照组(NS组),最后一次注射GH后1、2、24 h分批处死裸鼠,Western blotting检测SW1990细胞和移植瘤pSTAT5蛋白表达的变化.结果 所有胰腺癌细胞(SW1990、Cap-1、Colo、Mia、Aspc、P3、PANC1)均有pSTAT5表达.GH(50 ng/ml)刺激后5 min,SW1990细胞pSTAT5的表达量为0.57±0.05,较刺激前显著增高,10 min达到0.64±0.04,15 min很快下降至0.39±0.03,但直至1 h仍高于对照组(0.33±0.02对0.25±0.06),2 h后表达量为0.26±0.03,回落到基础水平.移植瘤pSTAT5表达无明显变化.结论 GH可迅速上调SW1990细胞的pSTAT5表达,但维持时间较短,而对胰腺癌移植瘤pSTAT5的表达无显著影响.
目的 檢測燐痠化轉錄激活因子5(pSTAT5)在7株胰腺癌細胞株中的錶達,觀察生長激素(GH)處理SW1990細胞及其移植瘤後pSTAT5錶達的變化,探討GH的分子作用機製.方法 體外培養人胰腺癌細胞株SW1990、Cap-1、Colo、Mia、Aspc、P3、PANC1,Western blotting檢測各株細胞的pSTAT5錶達;收集指數生長期的SW1990細胞接種于BALB/C裸鼠,成瘤後隨機分為GH組(瘤內註入GH 4 mg·kg-1·d-1,連續2週)和對照組(NS組),最後一次註射GH後1、2、24 h分批處死裸鼠,Western blotting檢測SW1990細胞和移植瘤pSTAT5蛋白錶達的變化.結果 所有胰腺癌細胞(SW1990、Cap-1、Colo、Mia、Aspc、P3、PANC1)均有pSTAT5錶達.GH(50 ng/ml)刺激後5 min,SW1990細胞pSTAT5的錶達量為0.57±0.05,較刺激前顯著增高,10 min達到0.64±0.04,15 min很快下降至0.39±0.03,但直至1 h仍高于對照組(0.33±0.02對0.25±0.06),2 h後錶達量為0.26±0.03,迴落到基礎水平.移植瘤pSTAT5錶達無明顯變化.結論 GH可迅速上調SW1990細胞的pSTAT5錶達,但維持時間較短,而對胰腺癌移植瘤pSTAT5的錶達無顯著影響.
목적 검측린산화전록격활인자5(pSTAT5)재7주이선암세포주중적표체,관찰생장격소(GH)처리SW1990세포급기이식류후pSTAT5표체적변화,탐토GH적분자작용궤제.방법 체외배양인이선암세포주SW1990、Cap-1、Colo、Mia、Aspc、P3、PANC1,Western blotting검측각주세포적pSTAT5표체;수집지수생장기적SW1990세포접충우BALB/C라서,성류후수궤분위GH조(류내주입GH 4 mg·kg-1·d-1,련속2주)화대조조(NS조),최후일차주사GH후1、2、24 h분비처사라서,Western blotting검측SW1990세포화이식류pSTAT5단백표체적변화.결과 소유이선암세포(SW1990、Cap-1、Colo、Mia、Aspc、P3、PANC1)균유pSTAT5표체.GH(50 ng/ml)자격후5 min,SW1990세포pSTAT5적표체량위0.57±0.05,교자격전현저증고,10 min체도0.64±0.04,15 min흔쾌하강지0.39±0.03,단직지1 h잉고우대조조(0.33±0.02대0.25±0.06),2 h후표체량위0.26±0.03,회락도기출수평.이식류pSTAT5표체무명현변화.결론 GH가신속상조SW1990세포적pSTAT5표체,단유지시간교단,이대이선암이식류pSTAT5적표체무현저영향.
Objective To investigate the expression of pSTAT5 in 7 pancreatic carcinoma cell lines,and the change of expression of pSTAT5 in pancreatic carcinoma cells SW1990 after growth hormone (GH) treatment, and explore its molecular mechanism. Methods Human pancreatic carcinoma cell lines (SW1990, Cap-1, Colo, Mia, AsPc, P3, PANC1) were cultured in vitro, and Western blotting was used to detect the expression of pSTAT5 in these cell lines. SW1990 in exponential growth phase was collected and nude Balb/c mice were inoculated with SW1990 cells. When tumors became palpable after inoculation, mice (normal saline group). 1 h, 2 h and 24 h after the last dose of GH treatment, the mice were sacrificed.Western blotting was used to detect the expression of pSTAT5 in SW1990 and inoculation tumor cells after GH injection. Results Positive expression of pSTAT5 was observed in all human pancreatic carcinoma cell lines (SW1990, Cap-1, Colo, Mia, Aspc, P3, PANC1). 5 minutes after GH (50 ng/ml) stimulation, the expression of pSTAT5 in SW1990 was 0.57 ±0.05, which was significantly increased; and it reached 0.64 ±0.04 at 10 minutes, then decreased to 0.39 ±0.03 at 15 minutes, however, it remained higher than that in the control group at 1 h (0.33 ± 0.02 vs 0.25 ± 0.06), and its expression at 2 h was 0.26 ± 0.03 and returned to the normal level. The expression of pSTAT5 in xenograft was not significantly changed. Conclusions GH could rapidly up-regulate the expression of pSTAT5 in SW1990 but the effect lasted for a relatively short period. GH had no significant effect on the expression of pSTAT5 in xenograft.
