CAJ | 학술논문

目的了解湖北省武汉地区啮齿动物自然感染汉坦病毒(HV)情况以及流行的基因型和亚型.方法 2000-2003年、2009-2011年秋冬季在武汉地区新洲、江夏区野外及居民区采用夹夜法捕鼠.对捕获的动物进行分类鉴定并取肺脏用间接免疫荧光法检测病毒抗原.抗原阳性的样本,采用RT-PCR方法扩增部分S片段核苷酸序列,构建系统发生树并进行基因分型.结果 2000-2003年捕获啮齿类动物437只,HV抗原阳性鼠肺标本24份,病毒携带率为5.49％.2009-2011年捕获啮齿类动物173只,HV抗原阳性鼠肺标本7份,病毒携带率为4.05％.褐家鼠为当地的优势鼠种.22份标本成功地用汉滩病毒(HTNV)、汉城病毒(SEOV)特异引物扩增部分S基因片段并测序.17只(13只褐家鼠,4只黑线姬鼠)鼠肺标本中扩增出SEOV部分S基因片段(nt 588～1147),分别属于第3亚型和2个新的基因亚型.5只黑线姬鼠的鼠肺标本中扩增出HTNV部分S基因片段(nt615- 1141),分别属于第7亚型和1个新的亚型.结论武汉地区流行的HV为SEOV和HTNV,并发现新的基因亚型,SEOV可能“溢出”感染黑线姬鼠.
목적 료해호북성무한지구교치동물자연감염한탄병독(HV)정황이급류행적기인형화아형.방법 2000-2003년、2009-2011년추동계재무한지구신주、강하구야외급거민구채용협야법포서.대포획적동물진행분류감정병취폐장용간접면역형광법검측병독항원.항원양성적양본,채용RT-PCR방법확증부분S편단핵감산서렬,구건계통발생수병진행기인분형.결과 2000-2003년포획교치류동물437지,HV항원양성서폐표본24빈,병독휴대솔위5.49％.2009-2011년포획교치류동물173지,HV항원양성서폐표본7빈,병독휴대솔위4.05％.갈가서위당지적우세서충.22빈표본성공지용한탄병독(HTNV)、한성병독(SEOV)특이인물확증부분S기인편단병측서.17지(13지갈가서,4지흑선희서)서폐표본중확증출SEOV부분S기인편단(nt 588～1147),분별속우제3아형화2개신적기인아형.5지흑선희서적서폐표본중확증출HTNV부분S기인편단(nt615- 1141),분별속우제7아형화1개신적아형.결론 무한지구류행적HV위SEOV화HTNV,병발현신적기인아형,SEOV가능“일출”감염흑선희서.
Objective To investigate the infection and genotype of hantaviruses in rodents from Wuhan area,Hubei province.Methods Rodents were trapped in fields and residential areas of Xinzhou and Jiangxia districts of Wuhan in autumn and winter seasons,from 2000 to 2003 and from 2009 to 2011.Trapped rodents were identified,and hantavirus antigens were detected in the lung tissues with indirect immunofluorescence assay (IFA).Partial S segment sequences were amplified with RT-PCR in hantavirus antigen positive samples and then sequenced.Phylogenetic tree was constructed to analyze the genetic characteristics of hantaviruses.Results From 2000 to 2003,437 rodents were trapped,with 24 (5.49％) lung tissues showed hantavirus antigen positive.From 2009 to 2011,173 rodents were trapped and 7 (4.05％) were hantavirus antigen positive.Rattus norvegicus were the dominant species of rodents.Partial S segment sequences were amplified from 22 samples with Hantaan and Seoul viruses specific primers and sequenced.Partial S segments of Seoul viruses (nucleotide 588-1147) were amplified from 17 rodents (13 R.norvegicus and 4 Apodemus agrarius).Seven of these sequences belonged to 3 genetic lineage,while two novel genetic lineages were formed by 9 and 1 sequences,respectively.Partial S segments of Hantaan viruses (nucleotide 615-1141 ) were amplified from 5 A.agrarius.One of these sequences belonged to 7 genetic lineages,and 4 sequences formed one novel genetic subtype.Conclusion Hantaan and Seoul viruses co-circulated in Wuhan area.Hubei province.Novel genetic lineages were identified in this study and Seoul virus might have caused spillover infection in A.agrarius.