CAJ | 학술논문

目的探讨血必净对心肺复苏后转录因子GATA-3和T-bet表达的调节作用.方法建立心肺复苏大鼠模型,30只SD大鼠动物随机(随机数字法)分为三组:假手术组(B组),仅进行麻醉和气管切开插管、血管穿刺,不进行窒息及心肺复苏;常规复苏组(C组),常规复苏+生理盐水4 mL/kg;血必净治疗组(D组),常规复苏+血必净4mL/kg.动态观察血清GATA-3和T-betmRNA表达及白介素-12(IL-12)、白介素-4(IL-4)、r-干扰素(IFN-γ)、肿瘤坏死因子-α(TNF-α)水平的变化.多组间均数比较采用单因素方差分析,方差齐则采用成组设计资料的t检验,两两比较用SNK法;如方差不齐,则采用随机区组设计的秩和检验(Wilcoxon检验),P<0.05为差异有统计学意义.结果 C,D组复苏后6 h与对照组、复苏后即刻比较,IL-12,IFN-γ,TNF-α水平均升高(P<0.01);C组复苏后6 h GATA表达下调(P>0.05),T-bel表达上调(P<0.01),GATA-3/T-bet下降(P<0.05).D组复苏后6 h T-bet表达上调(P>0.05),GATA表达上调(P<0.01),GATA-3/T-bet升高(P<0.05);D组复苏后6h与C组同时点比较,IL-12,IFN-γ,TNF-α水平低于C组(P<0.01),GATA-3表达上调、T-bet表达下调、GATA-3/T-bet升高均有统计学意义(P<0.01).结论心肺复苏后存在转录因子T-bet和GATA-3失衡表达,血必净可调节GATA-3/T-bet趋向平衡及细胞因子IL-12,IFN-γ,TNF-α水平.
목적 탐토혈필정대심폐복소후전록인자GATA-3화T-bet표체적조절작용.방법 건립심폐복소대서모형,30지SD대서동물수궤(수궤수자법)분위삼조:가수술조(B조),부진행마취화기관절개삽관、혈관천자,불진행질식급심폐복소;상규복소조(C조),상규복소+생리염수4 mL/kg;혈필정치료조(D조),상규복소+혈필정4mL/kg.동태관찰혈청GATA-3화T-betmRNA표체급백개소-12(IL-12)、백개소-4(IL-4)、r-간우소(IFN-γ)、종류배사인자-α(TNF-α)수평적변화.다조간균수비교채용단인소방차분석,방차제칙채용성조설계자료적t검험,량량비교용SNK법;여방차불제,칙채용수궤구조설계적질화검험(Wilcoxon검험),P<0.05위차이유통계학의의.결과 C,D조복소후6 h여대조조、복소후즉각비교,IL-12,IFN-γ,TNF-α수평균승고(P<0.01);C조복소후6 h GATA표체하조(P>0.05),T-bel표체상조(P<0.01),GATA-3/T-bet하강(P<0.05).D조복소후6 h T-bet표체상조(P>0.05),GATA표체상조(P<0.01),GATA-3/T-bet승고(P<0.05);D조복소후6h여C조동시점비교,IL-12,IFN-γ,TNF-α수평저우C조(P<0.01),GATA-3표체상조、T-bet표체하조、GATA-3/T-bet승고균유통계학의의(P<0.01).결론 심폐복소후존재전록인자T-bet화GATA-3실형표체,혈필정가조절GATA-3/T-bet추향평형급세포인자IL-12,IFN-γ,TNF-α수평.
Objective To investigate the effects of Xuebijing injection on the transcription factors GATA-3 and T-bet of rats after cardiopulmonary resuscitation (CPR). Method The rat models of CPR was made by using asphyxia method. Thirty SD rats were randomly (random number) divided into three groups at random: sham operation rats (B group), conventional CPR rats (C group) and Xuebijing (4 mL/kg) treated rats (D group). The levels of serum IL-12, IL-4, TNF-α and IFN-γ were measured by using ELISA. The expressions of T-bet and GATA-3 mRNA in serum were detected by RT-PCR. The analysis of variance was used to compare the means of different groups including t-test and Wilcoxon test. Results Compared with group B, the levels of serum IL-12, TNF-α and IFN-γ in group C and group D were significantly elevated after CPR for 6 hours (P<0.01). In group C, the expression of GATA-3 mRNA and GATA-3/T-bet decreased (both P < 0.05), while the expression of T-bet mRNA increased (P<0.01) after CPR for 6 hours. In group D, the expressions of GATA-3 mRNA and T-bet mRNA as well as GATA-3/T-bet increased after CPR for 6 hours. The levels of IL-12, IFN-γ and TNF-α in group D were lower than those in groupC (P<0.01). Compared with group C, the expression of GATA-3 mRNA and GATA-3/T-bet were significantly elevated and the expression of T-bet mRNA decreased in group D. ConclusionsThe transcription factors GATA-3 and T-bet may fail to get balance after CPR. The Xiebijing injectio can modulate the balance between GATA-3 and T-bet, and the levels of IL-12,IFN-γ and TNF-α.