骨髓增生异常综合征患者骨髓CD3+T细胞TET2和DLK1基因表达及其临床意义
골수증생이상종합정환자골수CD3+T세포TET2화DLK1기인표체급기림상의의
TET2 and DLK1 gene expression and their clinical significance in bone marrow CD3+ T cells of patients with myelodysplastic syndrome
  • 万方数据
    中华内科杂志 중화내과잡지
    CHINESE JOURNAL OF INTERNAL MEDICINE
    2012年 7期 543-546 ,共4页

    张薇%付蓉%王化泉%李丽娟%岳兰竹%刘惠%瞿文%梁勇%王国锦%王晓明%吴玉红%刘鸿%宋嘉%关晶%邢莉民%邵宗鸿

    장미%부용%왕화천%리려연%악란죽%류혜%구문%량용%왕국금%왕효명%오옥홍%류홍%송가%관정%형리민%소종홍

    骨髓增生异常综合征%CD3+T细胞%TET2基因%DLK1基因 骨髓增生異常綜閤徵%CD3+T細胞%TET2基因%DLK1基因
    골수증생이상종합정%CD3+T세포%TET2기인%DLK1기인
    Myelodysplastic syndrome%CD3+ T cells%TET2 gene%DLK1 gene
    目的 探讨骨髓增生异常综合征(MDS)患者骨髓CD3+T细胞TET2及DLK1基因的表达水平及其临床意义,为进一步阐明MDS细胞免疫异常的机制提供依据.方法 应用免疫磁珠分选骨髓CD3+T细胞,应用实时荧光定量PCR(qPCR)检测26例MDS患者和16例正常对照骨髓CD3+T细胞中TET2和DLK1 mRNA的表达水平,分析它们与临床特征之间的相关性.结果 MDS患者骨髓CD3+ T细胞中TET2 mRNA表达是正常对照组的(0.16±0.15)倍(P＜0.01)；TET2mRNA表达水平与血清补体C3呈显著正相关(r =0.404,P＜0.05)；MDS患者骨髓CD3+T细胞中DLK1 mRNA表达是正常对照组的(1.61±0.88)倍(P＜0.05)；依据染色体分组,染色体异常组DLK1表达水平是染色体正常对照组的(1.45±0.44)倍(P＜0.05)；DLK1 mRNA表达水平与骨髓原始细胞比例呈显著正相关(r =0.343,P＜0.05).结论 MDS患者骨髓CD3+T细胞中TET2 mRNA表达降低,DLK1 mRNA表达增高,提示CD3+T细胞有质的异常,这可能是导致机体免疫监视功能下降的机制之一.
    목적 탐토골수증생이상종합정(MDS)환자골수CD3+T세포TET2급DLK1기인적표체수평급기림상의의,위진일보천명MDS세포면역이상적궤제제공의거.방법 응용면역자주분선골수CD3+T세포,응용실시형광정량PCR(qPCR)검측26례MDS환자화16례정상대조골수CD3+T세포중TET2화DLK1 mRNA적표체수평,분석타문여림상특정지간적상관성.결과 MDS환자골수CD3+ T세포중TET2 mRNA표체시정상대조조적(0.16±0.15)배(P＜0.01)；TET2mRNA표체수평여혈청보체C3정현저정상관(r =0.404,P＜0.05)；MDS환자골수CD3+T세포중DLK1 mRNA표체시정상대조조적(1.61±0.88)배(P＜0.05)；의거염색체분조,염색체이상조DLK1표체수평시염색체정상대조조적(1.45±0.44)배(P＜0.05)；DLK1 mRNA표체수평여골수원시세포비례정현저정상관(r =0.343,P＜0.05).결론 MDS환자골수CD3+T세포중TET2 mRNA표체강저,DLK1 mRNA표체증고,제시CD3+T세포유질적이상,저가능시도치궤체면역감시공능하강적궤제지일.
    Objective To investigate the expression of TET2 and DLK1 mRNA in bone marrow CD3+ T cells of patients with myelodysplastic syndrome (MDS) and their clinical significance and to explore the potential mechanism of abnormal cell-mediated immunity.Methods CD3+ T cells were sorted by magnetic activated cell-sorting system.The expressions of TET2 and DLK1 mRNA in bone marrow CD3+ T cells from 26 MDS patients and 16 healthy controls were detected by fluorescence quantitative PCR.Results The expression of TET2 mRNA in CD3+ T cells was down-regulated in the MDS patients by (0.16 ±0.15) fold compared with the controls ( P ＜ 0.05 ).The expression of TET2 mRNA in CD3+ T cells of MDS patients was positively correlated with serum complement C3 ( r =0.404,P ＜ 0.05 ).The expression of DLK1 mRNA in CD3+ T cells was up-regulated in the MDS patients by (1.61 ±0.88) folds compared with the controls (P＜0.05).Grouped by the chromosomes,the patients with chromosome abnormalities presented significantly higher DLK1 mRNA level than those with normal chromosomes [ ( 1.45 ± 0.44 ) folds,P ＜0.05 ].The expression of DLK1 mRNA in CD3+ T cells of MDS patients was positively correlated with the proportion of bone marrow blasts ( r =0.343,P ＜ 0.05 ).Conclusions The mRNA expression of TET2 in CD3+ T cells of MDS patients was decreased while the mRNA expression of DLK1 was increased,which might decline the immune surveillance function.The findings would be useful for exploring the mechanism of immune tolerance.
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