CAJ | 학술논문

目的揭示成束蛋白(fascin)和细胞角蛋白14(CK14)在不同器官的相同或不同组织学类型癌组织中的表达规律,探讨其鉴别诊断的应用价值.方法构建450例不同器官的相同或不同组织学类型癌组织芯片,用免疫组化方法检测fascin和CK14的表达情况.结果 fascin在食管、肺、喉、宫颈和外生殖器的鳞癌中弥漫高表达,阳性率分别为90.0%、90.0%、96.7%、78.6%和89.7%,在肺腺癌、胃腺癌、结直肠腺癌、肝细胞癌、胰腺导管腺癌、乳腺浸润性导管癌、甲状腺乳头状癌、子宫宫内膜样腺癌、卵巢浆液性囊腺癌和肾透明细胞癌中表达阳性率分别为38.0%、23.3%、14.3%、10.3%、73.3%、13.3%、6.7%、60.0%、66.7%和10.0%;在鳞癌和其他组织学类型肿瘤中的表达差异有统计学意义(P＜0.001).CK14在食管、肺、喉、宫颈和外生殖器鳞癌中表达阳性率分别为76.7%、36.7%、83.3%、60.7%和96.3%,而在肺腺癌、胃腺癌、结直肠腺癌、肝细胞癌、胰腺导管腺癌、乳腺浸润性导管癌、甲状腺乳头状癌、子宫宫内膜样腺癌、卵巢浆液性囊腺癌和肾透明细胞癌中表达阳性率分别为13.3%、13.3%、20.7%、41.4%、46.7%、6.7%、40.0%、13.3%、20.0%和6.7%;在鳞癌和其他组织学类型肿瘤中的表达差异有统计学意义(P＜0.001).fascin与CK14联合,在鳞癌中任一蛋白阳性率,与在其他组织学类型肿瘤中的表达差异均有统计学意义(P＜0.001).结论 fascin和CK14在多器官鳞癌呈高表达,在其他组织学类型肿瘤呈较低表达.联合检测fascin和CK14的表达,可用于鳞癌的诊断和鉴别诊断.
목적 게시성속단백(fascin)화세포각단백14(CK14)재불동기관적상동혹불동조직학류형암조직중적표체규률,탐토기감별진단적응용개치.방법 구건450례불동기관적상동혹불동조직학류형암조직심편,용면역조화방법검측fascin화CK14적표체정황.결과 fascin재식관、폐、후、궁경화외생식기적린암중미만고표체,양성솔분별위90.0%、90.0%、96.7%、78.6%화89.7%,재폐선암、위선암、결직장선암、간세포암、이선도관선암、유선침윤성도관암、갑상선유두상암、자궁궁내막양선암、란소장액성낭선암화신투명세포암중표체양성솔분별위38.0%、23.3%、14.3%、10.3%、73.3%、13.3%、6.7%、60.0%、66.7%화10.0%;재린암화기타조직학류형종류중적표체차이유통계학의의(P＜0.001).CK14재식관、폐、후、궁경화외생식기린암중표체양성솔분별위76.7%、36.7%、83.3%、60.7%화96.3%,이재폐선암、위선암、결직장선암、간세포암、이선도관선암、유선침윤성도관암、갑상선유두상암、자궁궁내막양선암、란소장액성낭선암화신투명세포암중표체양성솔분별위13.3%、13.3%、20.7%、41.4%、46.7%、6.7%、40.0%、13.3%、20.0%화6.7%;재린암화기타조직학류형종류중적표체차이유통계학의의(P＜0.001).fascin여CK14연합,재린암중임일단백양성솔,여재기타조직학류형종류중적표체차이균유통계학의의(P＜0.001).결론 fascin화CK14재다기관린암정고표체,재기타조직학류형종류정교저표체.연합검측fascin화CK14적표체,가용우린암적진단화감별진단.
Objective To investigate and analyze the expression of fascin and CK14 in multiple histological types of cancer and to explore the potential value of the two proteins as markers in diagnosis and differential diagnosis of various cancer types. Methods Tissue microarray containing esophageal squamous cell carcinoma (SCC), lung SCC, larynx SCC, uterine cervical SCC, SCC of external genital organs, lung adenocarcinoma, gastric adenocarcinoma, colorectal adenocarcinoma, heptocellular carcinoma, pancreatic ductal adenocarcinoma, breast infiltrating ductal carcinoma, thyroid papillary carcinoma, uterine endometrioid adenocarcinoma, ovarian serous adenocarcinoma and renal clear cell carcinoma, 30 cases each, as well as corresponding normal controls was constructed. The expression of fascin and CK14 among different types of carcinoma and corresponding normal controls was detected by immunohistochemistry.Results In normal esophagus, bronchus, larynx, uterine cervix and skin, fascin was mainly expressed in the basal cells or reserve cells, but the expression was diffuse in esophageal SCC, lung SCC, larynx SCC,uterine cervical SCC and SCC of external genital organs, with a positive rate of 90.0%, 90.0%, 96.7%,78.6% and 89.7%, respectively. In the normal tissue of other organs, except breast and uterine endometrium, fascin was negative. In lung adenocarcinoma, gastric adenocarcinoma, colorectal adenocarcinoma, hepatocellular carcinoma, pancreatic ductal adenocarcinoma, breast infiltrating dutal adenocarcinoma, thyroid papillary carcinoma, uterine endometrioid adenocarcinoma, ovarian serous adenocarcinoma and renal clear cell carcinoma, the positive rates were 38.0%, 23.3%, 14.3%, 10.3%,73.3%, 13.3%, 6.7%, 60.0%, 66.7% and 10.0%, respectively. The difference between fascin expression in SCC and in other histological types was statistically significant (P ＜0.001 ). CK14 was mainly expressed in the basal cells, reserve cells or myoepithelia of normal tissues. The positive rates of CK14 were 76.7%, 36.7%, 83.3%, 60.7% and 96.3% in esophageal SCC, lung SCC, larynx SCC, uterine cervical SCC and SCC of external genital organs, respectively. It was weak and focal in lung adenocarcinoma, gastric adenocarcinoma, colorectal adenocarcinoma, hepatocellular carcinoma, pancreatic ductal adenocarcinoma,breast infiltrating dutal adenocarcinoma, thyroid papillary carcinoma, uterine endometrioid adenocarcinoma,ovarian serous adenocarcinoma, and renal clear cell carcinoma, with a positive rate of 13. 3%, 13. 3%,20.7%,41.4%, 46.7%, 6.7%, 40.0%, 13.3%, 20.0% and 6.7%, respectively. The difference between CK14 expression in SCC and in other histological types was statistically significant (P＜0.001).The difference between co-expression of fascin/CKl4 in SCC and in other histological types was also statistically significant (P＜0.001). Conclusion Fascin and CK14 are highly expressed in SCC,compared with other histological types of carcinoma. Combination of fascin and CK14 should be a valuable marker in diagnosis and differential diagnosis of carcinoma.