农业科学与技术(英文版)
農業科學與技術(英文版)
농업과학여기술(영문판)
AGRICULTURAL SCIENCE & TECHNOLOGY
2008年
4期
87-91,152
,共6页
刘凤军%张玉玲%杨自军%陈兴启%孙达权%王国华%张涌
劉鳳軍%張玉玲%楊自軍%陳興啟%孫達權%王國華%張湧
류봉군%장옥령%양자군%진흥계%손체권%왕국화%장용
Somatic cell nuclear transfer%Transgene%Human lactoferrin%Goat fetal fibroblasts%Mammary gland epithelial cells
[Objective] The aim of this study is to understand the effects of donor cell type, embryo stage, number and transfer position on the efficiency of goat transgenic clone. [Method] Using somatic cell nuclear transfer technology, the single goat fetal fibroblasts (GFF) and mammary gland epithelial cells (GMGE) harboring human lactoferrin (hLF) gene were transferred to the enucleated oocyte. Reconstructed karyoplast-cytoplast couplets were fused, activated, and cultured in vitro. Embryos at 2-8 cell stage were transferred into oviduct of synchronized recipients, and blastocysts were transferred into uterine horn. [Result] The pregnancy rate was similar between GFF and GMGE (oviduct transfer:26.47% vs. 20.00%), and between oviduct transfer and uterine horn transfer (26.47% vs. 25.00%) for GFF group; pregnancy rate in the group with the mean number of embryo transferred per recipient of 21.2 was significantly higher than in those the 5.93 group and 9.64 group (40.00% vs. 26.67% and 21.43%). [Conclusion] These results indicate that pregnancy rate of goat transgenic clone couldn't be affected by donor cell type, embryo stage and transfer position but be done by the number of embryo transferred per recipient. In addition, the study also suggests the feasibility of making transgenic goat using GMGE as donor cells.