CAJ | 학술논문

为了提高大丽轮枝菌(Verticillium dahliae)拮抗细菌多粘芽孢杆菌(Bacillus polymyxa)7-4菌株的发酵产抗菌蛋白量,采用单因素法对B.polymyxa 7-4菌株发酵培养基所需的碳源、氮源、无机盐进行了筛选,并采用正交试验与单因素试验对7-4号菌株的发酵培养基组成及培养条件进行了优化,最终确定最适发酵培养基及培养条件为:葡萄糖5.0%,大豆蛋白胨5.0%,MnSO4 0.02%,MgSO4·7H2O 0.10%,培养基初始pH 7.5,种龄10～12 h,30℃摇床培养48 h.经过优化抑菌圈直径增加了50.4%.
위료제고대려륜지균(Verticillium dahliae)길항세균다점아포간균(Bacillus polymyxa)7-4균주적발효산항균단백량,채용단인소법대B.polymyxa 7-4균주발효배양기소수적탄원、담원、무궤염진행료사선,병채용정교시험여단인소시험대7-4호균주적발효배양기조성급배양조건진행료우화,최종학정최괄발효배양기급배양조건위:포도당5.0%,대두단백동5.0%,MnSO4 0.02%,MgSO4·7H2O 0.10%,배양기초시pH 7.5,충령10～12 h,30℃요상배양48 h.경과우화억균권직경증가료50.4%.
In order to increase the quantity of antifungal protein from Bacillus polymyxa 7-4, the strain was cultured under different conditions. Through single factor experiments, the suitable carbon source, nitrogen source and inorganic salt were chosen. The optimal composition of media was determined by orthogonal experiments. The optimization of fermentation factors, such as initial pH, fermentation temperature and fermentation time, was made by single factor experiments. By orthogonal design, the optimal proportions of fermentation single factor experiments, the optimal fermentation condition was initial pH 7.5 and inoculum age of 10～12 hours, shaking flask fermentation temperature is 30℃ for 48 hours under the conditions, the most quantity of antifungal protein was obtained. After optimization, diameter of inhibition zone was increased by 50.4%.