临床肿瘤学杂志
臨床腫瘤學雜誌
림상종류학잡지
CHINESE CLINICAL ONCOLOGY
2009年
7期
602-605
,共4页
紫杉醇%胃腺癌细胞%环氧化酶-2%P-糖蛋白%多药耐药
紫杉醇%胃腺癌細胞%環氧化酶-2%P-糖蛋白%多藥耐藥
자삼순%위선암세포%배양화매-2%P-당단백%다약내약
Paclitaxl%Gaticancacinmacll%CX-2%P-gp%Multi-ugitanc(M)
目的:观察紫杉醇及环氧化酶-2(COX-2)选择性抑制剂塞来昔布(Celecoxib)对人胃腺癌细胞株BGC-823 COX-2及P-糖蛋白(P-gp)表达的影响,探讨COX-2在化疗药物诱发多药耐药(MDR)产生机制中的作用.方法:采用MTT法检测紫杉醇不同剂量、不同时间点及塞来昔布不同剂量对胃腺癌细胞株BGC-823生长的影响,在此基础上采用Western blot方法检测一定剂量范围内某一时间点的紫杉醇对BGC-823 COX-2、P-gp表达的影响及联合塞来昔布后两种蛋白表达的变化.结果:紫杉醇对胃腺癌细胞株BGC-823 的生长为细胞毒作用,呈时间和剂量依赖性.在一定剂量范围内和某一时间点,随着紫杉醇剂量逐渐升高,BGC-823的COX-2、P-gp表达均呈上升趋势;且这两种蛋白在联合塞来昔布应用后表达均呈下降趋势.结论:紫杉醇可诱导胃腺癌细胞株BGC-823 COX-2及P-gp蛋白的表达,这种表达能被塞来昔布所抑制.COX-2表达的诱导在紫杉醇诱导的P-gp表达中起着重要作用.
目的:觀察紫杉醇及環氧化酶-2(COX-2)選擇性抑製劑塞來昔佈(Celecoxib)對人胃腺癌細胞株BGC-823 COX-2及P-糖蛋白(P-gp)錶達的影響,探討COX-2在化療藥物誘髮多藥耐藥(MDR)產生機製中的作用.方法:採用MTT法檢測紫杉醇不同劑量、不同時間點及塞來昔佈不同劑量對胃腺癌細胞株BGC-823生長的影響,在此基礎上採用Western blot方法檢測一定劑量範圍內某一時間點的紫杉醇對BGC-823 COX-2、P-gp錶達的影響及聯閤塞來昔佈後兩種蛋白錶達的變化.結果:紫杉醇對胃腺癌細胞株BGC-823 的生長為細胞毒作用,呈時間和劑量依賴性.在一定劑量範圍內和某一時間點,隨著紫杉醇劑量逐漸升高,BGC-823的COX-2、P-gp錶達均呈上升趨勢;且這兩種蛋白在聯閤塞來昔佈應用後錶達均呈下降趨勢.結論:紫杉醇可誘導胃腺癌細胞株BGC-823 COX-2及P-gp蛋白的錶達,這種錶達能被塞來昔佈所抑製.COX-2錶達的誘導在紫杉醇誘導的P-gp錶達中起著重要作用.
목적:관찰자삼순급배양화매-2(COX-2)선택성억제제새래석포(Celecoxib)대인위선암세포주BGC-823 COX-2급P-당단백(P-gp)표체적영향,탐토COX-2재화료약물유발다약내약(MDR)산생궤제중적작용.방법:채용MTT법검측자삼순불동제량、불동시간점급새래석포불동제량대위선암세포주BGC-823생장적영향,재차기출상채용Western blot방법검측일정제량범위내모일시간점적자삼순대BGC-823 COX-2、P-gp표체적영향급연합새래석포후량충단백표체적변화.결과:자삼순대위선암세포주BGC-823 적생장위세포독작용,정시간화제량의뢰성.재일정제량범위내화모일시간점,수착자삼순제량축점승고,BGC-823적COX-2、P-gp표체균정상승추세;차저량충단백재연합새래석포응용후표체균정하강추세.결론:자삼순가유도위선암세포주BGC-823 COX-2급P-gp단백적표체,저충표체능피새래석포소억제.COX-2표체적유도재자삼순유도적P-gp표체중기착중요작용.
Objective:To observe the effect of paclitaxel and a selective cyclooxygenase-2(COX-2) inhibitor named celecoxib on the expression of COX-2 and P-giycoprotein(P-gp) in human gastic adenocarcinoma cell line BGC823, and to study the role that COX-2 played in the mechanism of chemotherapy-induced multi-drug resistance(MDR). Methods:The effects of paclitaxel with various doses and time points on BGC823 cell growth was assessed by MTT assay, and so did the effects of celecoxib with different doses. The effect of a dose-ranging paclitaxel in at one time point and the change of combining celecoxib with paclitaxel on the COX-2 and P-gp protein expression was detected by Western blot. Results:Paclitaxel had the effect of cellular toxicity on BGC823 growth, which in dose-dependent and time-dependent manners. With a limited ranging dose and at certain time piont, when the dose of paclitaxel increasing gradually, the expression of both COX-2 and P-gp showed rising trend in BGC823 cell line. And the expression of the two proteins could indicate a downtrend after combined with celecoxib. Conclusion:Paclitaxel can induce the expression of COX-2 and P-gp increased in BGC823 at the same time, and the enhanced expression could be inhibited by celecoxib. The inducing of COX-2 expression should take a important part in paclitaxel-induced expression of P-gp.
