CAJ | 학술논문

目的探讨常通口服液(CTOL)对体外培养的正常腹膜成纤维细胞(NF)及粘连腹膜成纤维细胞(AF)增殖的影响.方法 (1)药物血清的制备:雄性SD大鼠20只,随机分为4组:正常血清组,CTOL低、中、高剂量组.大鼠灌服相应剂量的药物7d后,腹主动脉采血,分离药物血清.(2)成纤维细胞的体外培养:采用组织块贴壁培养法进行成纤维细胞培养,本实验所用细胞为第3～8代.(3)成纤维细胞的增殖:细胞以5×10~3/孔接种到96孔培养板,分别加入CTOL低、中、高剂量的含药血清和空白对照血清,培养24、48、72、96h,MTT法观察细胞增殖情况.结果药物剂量与吸光度值之间存在负相关性,同时药物剂量与抑制率之间存在正相关性.与NF正常血清对照组比较,CTOL各剂量组在作用24、48h时,其OD值均无显著性变化(P>0.05),但当作用72、96 h时,其OD值均呈显著性降低(P<0.01);与AF正常血清对照组比较,CTOL低、中、高剂量组药物血清作用24、48、72、96h,其0D值均呈显著性降低(P<0.05),同一时间点比较,CTOL低剂量对AF的抑制率比对NF的抑制率高,但无统计学差异.CTOL中剂量在作用96h,对AF的抑制率与NF的抑制率比较,有统计学差异(P<0.05).CTOL高剂量在作用72、96 h,对AF的抑制率与NF的抑制率比较,均有统计学差异(P<0.05).结论 CTOL能够抑制体外培养的NF和AF增殖.CTOL对成纤维细胞增殖的抑制率之间存在剂量依赖关系,而且,药物血清对AF作用更敏感.
목적 탐토상통구복액(CTOL)대체외배양적정상복막성섬유세포(NF)급점련복막성섬유세포(AF)증식적영향.방법 (1)약물혈청적제비:웅성SD대서20지,수궤분위4조:정상혈청조,CTOL저、중、고제량조.대서관복상응제량적약물7d후,복주동맥채혈,분리약물혈청.(2)성섬유세포적체외배양:채용조직괴첩벽배양법진행성섬유세포배양,본실험소용세포위제3～8대.(3)성섬유세포적증식:세포이5×10~3/공접충도96공배양판,분별가입CTOL저、중、고제량적함약혈청화공백대조혈청,배양24、48、72、96h,MTT법관찰세포증식정황.결과 약물제량여흡광도치지간존재부상관성,동시약물제량여억제솔지간존재정상관성.여NF정상혈청대조조비교,CTOL각제량조재작용24、48h시,기OD치균무현저성변화(P>0.05),단당작용72、96 h시,기OD치균정현저성강저(P<0.01);여AF정상혈청대조조비교,CTOL저、중、고제량조약물혈청작용24、48、72、96h,기0D치균정현저성강저(P<0.05),동일시간점비교,CTOL저제량대AF적억제솔비대NF적억제솔고,단무통계학차이.CTOL중제량재작용96h,대AF적억제솔여NF적억제솔비교,유통계학차이(P<0.05).CTOL고제량재작용72、96 h,대AF적억제솔여NF적억제솔비교,균유통계학차이(P<0.05).결론 CTOL능구억제체외배양적NF화AF증식.CTOL대성섬유세포증식적억제솔지간존재제량의뢰관계,이차,약물혈청대AF작용경민감.
Objective To investigate the effect of Changtong oral liquid (CTOL) on the proliferation of cultured fibroblasts derived from normal peritoneum (NFs) and adhesive peritoneum (AFs) of rats. Methods Twenty male SD rats were randomized into 4 groups, including a normal serum group and 3 CTOL groups with CTOL treatment at low, medium or high doses. Serum samples were obtained from the abdominal arteries of the rats after oral treatment with CTOL for 7 days. The fibroblasts were isolated from the peritoneum by means of tissue culture, and the passage 3-8 cells were cultured with the sera of the normal control and CTOL groups for 24, 48, 72 and 96 h. MTT assay was used to observe the proliferation of the fibroblasts. Results The dose of CTOL was inversely correlated to the absorbance but positively to the growth inhibition rates.Compared with the NFs cultured in normal control rat serum, the NFs in serum from CTOL groups showed no obviously changes in the absorbance at 24 and 48 h, but displayed significant reduction at 72 and 96 h (P<0.01). Compared with the AFs in normal rat serum, the AFs in the 3 CTOL groups all showed significantly decreased absorbance at 24, 48, 72 and 96 h (P<0.05). At the same time point, the inhibition rate of AFs in low-dose CTOL group showed no significant difference from that in the normal control group, but CTOL at a medium dose resulted in a significantly higher inhibition rate of AFs at 72 h (P<0.05). High-dose CTOL produced significant differences in the inhibition rates of AFs and NFs (P<0.05). Conclusion CTOL can inhibit the proliferation of AFs and NFs in vitro. AFs appear to be more sensitive to CTOL, which has a dose-dependent inhibitory effect of AF proliferation.