复旦学报(医学版)
複旦學報(醫學版)
복단학보(의학판)
JOURNAL OF FUDAN UNIVERSITY
2010年
2期
167-171
,共5页
恩度%胃癌%Bax%Bcl-2
恩度%胃癌%Bax%Bcl-2
은도%위암%Bax%Bcl-2
endostatin%gastric carcinoma%Bax%Bcl-2
目的 探讨恩度对人胃癌裸鼠移植瘤的抑制效应及机制.方法 用体外培养的SGC-7901人胃癌细胞细胞制作荷瘤裸鼠,研究恩度作用对肿瘤生长的抑制效应.分别采用实时荧光定量PCR法、免疫组织化学染色法研究肿瘤组织中Bax及Bcl-2在mRNA及蛋白水平表达的变化.结果 恩度治疗组肿瘤体积明显小于对照组(P<0.05),实验第7、10、13、16、19天恩度的抑瘤率分别为26.2%、47.7%、38.9%、45.8%、51.7%.实时荧光定量PCR法结果表明,恩度组作用后Bcl-2在mRNA水平的表达显著低于对照组(P<0.05),但对Bax的表达无影响(P>0.05).免疫组织化学结果与实时荧光定量PCR法结果一致.结论 恩度可显著抑制裸鼠SGC-7901人胃癌的生长,可能是通过降低Bcl-2的表达而发挥其杀伤效应.
目的 探討恩度對人胃癌裸鼠移植瘤的抑製效應及機製.方法 用體外培養的SGC-7901人胃癌細胞細胞製作荷瘤裸鼠,研究恩度作用對腫瘤生長的抑製效應.分彆採用實時熒光定量PCR法、免疫組織化學染色法研究腫瘤組織中Bax及Bcl-2在mRNA及蛋白水平錶達的變化.結果 恩度治療組腫瘤體積明顯小于對照組(P<0.05),實驗第7、10、13、16、19天恩度的抑瘤率分彆為26.2%、47.7%、38.9%、45.8%、51.7%.實時熒光定量PCR法結果錶明,恩度組作用後Bcl-2在mRNA水平的錶達顯著低于對照組(P<0.05),但對Bax的錶達無影響(P>0.05).免疫組織化學結果與實時熒光定量PCR法結果一緻.結論 恩度可顯著抑製裸鼠SGC-7901人胃癌的生長,可能是通過降低Bcl-2的錶達而髮揮其殺傷效應.
목적 탐토은도대인위암라서이식류적억제효응급궤제.방법 용체외배양적SGC-7901인위암세포세포제작하류라서,연구은도작용대종류생장적억제효응.분별채용실시형광정량PCR법、면역조직화학염색법연구종류조직중Bax급Bcl-2재mRNA급단백수평표체적변화.결과 은도치료조종류체적명현소우대조조(P<0.05),실험제7、10、13、16、19천은도적억류솔분별위26.2%、47.7%、38.9%、45.8%、51.7%.실시형광정량PCR법결과표명,은도조작용후Bcl-2재mRNA수평적표체현저저우대조조(P<0.05),단대Bax적표체무영향(P>0.05).면역조직화학결과여실시형광정량PCR법결과일치.결론 은도가현저억제라서SGC-7901인위암적생장,가능시통과강저Bcl-2적표체이발휘기살상효응.
Objective To investigate the inhibitory effect and the mechanism of endostatin on human gastric carcinoma model in nude mice. Methods Athymic mice were injected by in vitro-cultured SGC-7901 human carcinoma cell strain to observe the inhibitory effect of endostatin. Real time fluorescent quantitative PCR and immunohistochemistry stain were used to quantify the expression levels of Bcl-2 and Bax genes both on mRNA and protein levels. Results The average tumor volume in endostatin group was statistically smaller than control group (P<0.05). The inhibitory rate on the 7~(th), 10~(th), 13~(th), 16~(th) and 19th day was 26.2%, 47.7%, 38.9%, 45.8% and 51.7%, respectively. The results of real time fluorescent quantitative PCR demonstrated that mRNA expression of Bcl-2 in endostatin group was obviously lower than it in control group (P<0.05), however, the expression level of Bax had no statistical difference between these two groups (P>0.05). The results of immonohistochemistry were consistent with PCR. Conclusions Endostatin can inhibit the proliferation of SGC-7901 human gastric carcinoma in athymic mouse model, and the mechanism involves the suppression of Bcl-2 expression.
