中国医师杂志
中國醫師雜誌
중국의사잡지
JOURNAL OF CHINESE PHYSICIAN
2010年
11期
1452-1456
,共5页
载脂蛋白B类/遗传学%多态现象,遗传%血脂异常/遗传学/代谢%脂类
載脂蛋白B類/遺傳學%多態現象,遺傳%血脂異常/遺傳學/代謝%脂類
재지단백B류/유전학%다태현상,유전%혈지이상/유전학/대사%지류
Apolipoproteins B/GE%Polymorphism,genetic%Dyslipidemias/GE/ME%Lipids
目的 分析载脂蛋白B(apolipoprotein B,apo B)基因EcoRI及XbaI位点多态性与新疆石河子地区汉族人群血脂异常的关系.方法 采用聚合酶链式反应-限制性片段长度多态性分析法检测150例血脂异常患者和150例正常对照者的apoB基因EcoRI及XbaI位点多态性,并测定所有样本血清总胆固醇(total cholesterol,TC),三酰甘油(triglyceride,TG),低/高密度脂蛋白胆固醇(low/high density lipoprotein cholesterol,LDL-C/HDL-C),载脂蛋白AI\(apolipoproteinAI,apoAI)和apoB水平.结果 (1)血脂异常组E+E-/E-E-基因型及E-等位基因含量(分别为37.3%和19%)均高于对照组(分别为12.7%和6.3%),两组中携带E+E-/E-E-基因型者TC、TG和LDL-C均高于携带E+E+基因型者(P＜0.01);(2)血脂异常组X+X-/X+X+基因型及X+等位基因含量(分别为20.7%和11%)均高于对照组(分别为8%和4%),两组中携带X+X-/X+X+基因型者TC、TG、LDL-C和apoB水平高于同组携带X-X-基因型者(P＜0.05).结论 apoB基因EcoRI及XbaI位点多态性与新疆石河子地区汉族人群血脂异常有关,E-和X+等位基因可能是血脂异常的易感危险因素.
目的 分析載脂蛋白B(apolipoprotein B,apo B)基因EcoRI及XbaI位點多態性與新疆石河子地區漢族人群血脂異常的關繫.方法 採用聚閤酶鏈式反應-限製性片段長度多態性分析法檢測150例血脂異常患者和150例正常對照者的apoB基因EcoRI及XbaI位點多態性,併測定所有樣本血清總膽固醇(total cholesterol,TC),三酰甘油(triglyceride,TG),低/高密度脂蛋白膽固醇(low/high density lipoprotein cholesterol,LDL-C/HDL-C),載脂蛋白AI\(apolipoproteinAI,apoAI)和apoB水平.結果 (1)血脂異常組E+E-/E-E-基因型及E-等位基因含量(分彆為37.3%和19%)均高于對照組(分彆為12.7%和6.3%),兩組中攜帶E+E-/E-E-基因型者TC、TG和LDL-C均高于攜帶E+E+基因型者(P＜0.01);(2)血脂異常組X+X-/X+X+基因型及X+等位基因含量(分彆為20.7%和11%)均高于對照組(分彆為8%和4%),兩組中攜帶X+X-/X+X+基因型者TC、TG、LDL-C和apoB水平高于同組攜帶X-X-基因型者(P＜0.05).結論 apoB基因EcoRI及XbaI位點多態性與新疆石河子地區漢族人群血脂異常有關,E-和X+等位基因可能是血脂異常的易感危險因素.
목적 분석재지단백B(apolipoprotein B,apo B)기인EcoRI급XbaI위점다태성여신강석하자지구한족인군혈지이상적관계.방법 채용취합매련식반응-한제성편단장도다태성분석법검측150례혈지이상환자화150례정상대조자적apoB기인EcoRI급XbaI위점다태성,병측정소유양본혈청총담고순(total cholesterol,TC),삼선감유(triglyceride,TG),저/고밀도지단백담고순(low/high density lipoprotein cholesterol,LDL-C/HDL-C),재지단백AI\(apolipoproteinAI,apoAI)화apoB수평.결과 (1)혈지이상조E+E-/E-E-기인형급E-등위기인함량(분별위37.3%화19%)균고우대조조(분별위12.7%화6.3%),량조중휴대E+E-/E-E-기인형자TC、TG화LDL-C균고우휴대E+E+기인형자(P＜0.01);(2)혈지이상조X+X-/X+X+기인형급X+등위기인함량(분별위20.7%화11%)균고우대조조(분별위8%화4%),량조중휴대X+X-/X+X+기인형자TC、TG、LDL-C화apoB수평고우동조휴대X-X-기인형자(P＜0.05).결론 apoB기인EcoRI급XbaI위점다태성여신강석하자지구한족인군혈지이상유관,E-화X+등위기인가능시혈지이상적역감위험인소.
Objective To explore the association of apoB gene polymorphisms with dyslipidemia and lipid levels in Xinjiang Shihezi Han Chinese. Methods 150 dyslipidemia patients and 150 normal pople were involved in this study. EcoRI and XbaI polymorphisms of apolipoprotein B was analyzed by PCR-restriction fragment length polymorphism. The levels of plasma total cholesterol, triglyceride, low density lipoprotein cholesterol, high density lipoprotein cholesterol, apoAI and apoB were determined. Results The frequency of E + E -/E - E - genotype and E-Allele(37.3% and 19% ) in dyslipidemia group was significantly higher than that in controls( 12. 7% and 6. 3% ). The levels of TC, TG and LDL-C in the E +E -/E - E - gene type were significantly higher than those of the E + E + gene type in each group ( P ＜0. 01 ). The frequency of X + X -/X + X + genotype and X + Allele( 20. 7% and 1 1% ) in dyslipidemia group was significantly higher than that in controls (8% and 4% ). The levels ofTC, TG, LDL-C and apoB in the X + X -/X + X + gene type patients were significantly higher than those in the X - X - gene type patients in every group ( P ＜ 0. 05 ). Conclusion The EcoRI and XbaI polymorphism of ApoB gene was related to dyslipidemia in population of Xinjiang Shihezi Han Chinese, and the E - and X + Allele may be the genetic risk factors for dyslipidemia.
