CAJ | 학술논문

目的研究低强度微波对受γ射线照射小鼠的辐射防护作用及其可能机制.方法 96只雄性昆明种小鼠随机分为对照组、微波照射组(微波组,120μW/cm2,900 MHz,比吸收率为0.4 W/kg)、电离组(5 Gy γ射线照射)及微波与γ射线复合照射组(复合组,120μW/cm2微波+5 Gy γ射线照射),每组24只.微波组与复合组小鼠先接受功率密度为120 μW/cm2的微波照射,1 h/d,持续14 d,第15天给予电离组和复合组5 Gy 60Co γ射线一次性全身照射.于照射后3、6、9及12 d处死动物,固定胸骨及脾脏,作病理观察,流式细胞仪检测受照射小鼠的脾细胞凋亡率,同时测定血清中抗氧化酶和脂质过氧化物水平.结果骨髓病理切片显示,单独γ射线与复合照射均可导致骨髓组织经历典型的凋亡坏死、空虚、再生修复和恢复4个阶段的病理改变,但复合组的病变轻于电离组,恢复也更快;脾损伤病变过程与骨髓造血组织基本相似,复合组病变轻于电离组.流式细胞仪结果显示,照后6、9 d复合组脾细胞凋亡率(分别为23.02%±15.18%、25.37%±11.62%)明显低于电离组,差异有统计学意义(P<0.05);氧化损伤检测表明,复合组照后3、9、12 d血清中超氧化物歧化酶(SOD)活力较电离组明显升高,6、9、12d丙二醛(MDA)含量明显降低,差异均有统计学意义(P<0.05).结论特定强度的微波辐射具有一定的辐射防护作用,其作用机制可能与抑制辐射诱导的造血细胞凋亡和抗氧化损伤作用有关.
목적 연구저강도미파대수γ사선조사소서적복사방호작용급기가능궤제.방법 96지웅성곤명충소서수궤분위대조조、미파조사조(미파조,120μW/cm2,900 MHz,비흡수솔위0.4 W/kg)、전리조(5 Gy γ사선조사)급미파여γ사선복합조사조(복합조,120μW/cm2미파+5 Gy γ사선조사),매조24지.미파조여복합조소서선접수공솔밀도위120 μW/cm2적미파조사,1 h/d,지속14 d,제15천급여전리조화복합조5 Gy 60Co γ사선일차성전신조사.우조사후3、6、9급12 d처사동물,고정흉골급비장,작병리관찰,류식세포의검측수조사소서적비세포조망솔,동시측정혈청중항양화매화지질과양화물수평.결과 골수병리절편현시,단독γ사선여복합조사균가도치골수조직경력전형적조망배사、공허、재생수복화회복4개계단적병리개변,단복합조적병변경우전리조,회복야경쾌;비손상병변과정여골수조혈조직기본상사,복합조병변경우전리조.류식세포의결과 현시,조후6、9 d복합조비세포조망솔(분별위23.02%±15.18%、25.37%±11.62%)명현저우전리조,차이유통계학의의(P<0.05);양화손상검측표명,복합조조후3、9、12 d혈청중초양화물기화매(SOD)활력교전리조명현승고,6、9、12d병이철(MDA)함량명현강저,차이균유통계학의의(P<0.05).결론 특정강도적미파복사구유일정적복사방호작용,기작용궤제가능여억제복사유도적조혈세포조망화항양화손상작용유관.
Objective To investigate radiation protection and possible mechanisms of low intensity microwave on γ-ray exposed mice. Methods 96 healthy Kunming mice were randomly divided into the fol-lowing four groups: normal control, microwave (120 μW/cm2, 900 MHz), γ-ray irradiation (5 Gy), combined ex-posure of microwave and γ-ray(120 μW/cm2 +5 Gy). The microwave group and combined group were exposed to 120 μW/cm2 microwave firstly, 1 h/d, for 14 days. Then the ionization and combined group were exposed to 5 Gy 60Co γ-ray irradiation on the 15th day. Animals were sacrificed on the third,6th,9th and 12th day after ir-radiation. The sternum and spleen paraffin section were produced, and the histological changes were observed. Apoptosis rate of mice splenic cells in each group was examined by flow cytometry, and serum concentration of antioxidase and lipid peroxide was detected at the same time. Results Bone marrow was obviously injured ei-ther by radiation or microwave exposure, characterized by undergoing four-phase lesions, namely apoptosis-necrosis, void, regeneration and recovery phase. Compared with the γ-ray group, the pathological changes in combined group were slighter and the recovery was quicker. The pathological injuries of spleen were similar to that of bone marrow. Injuries in the combined group were slighter than γ-ray group. It showed that apoptosis rate of splenic cells in combined group was significantly lower on the 6th and 9th day after γ-ray radiation (23.02%±15.18%, 25.37%±11.62% respectively) from FCM results. Assays of oxidative damages suggested that serum superoxide dismutase (SOD)level in combined group increased while lipid peroxide level decreased significantly(P<0.05 ). Conclusion Low intensity microwave may exert protection effects on injuries induced by ionizing radiation. The underlying mechanisms might be related with suppression on the hematopoietic cells apoptosis induced by γ-ray radiation, inhibition of oxidative damages, and thus enhanced reconstruction of the hematopoietic system.