中华创伤骨科杂志
中華創傷骨科雜誌
중화창상골과잡지
CHINESE JOURNAL OF ORTHOPAEDIC TRAUMA
2009年
1期
45-50
,共6页
韩昕光%毕郑钢%王东奎%杨成林%刘伟
韓昕光%畢鄭鋼%王東奎%楊成林%劉偉
한흔광%필정강%왕동규%양성림%류위
骨折,不愈合%骨形态发生蛋白质类%核糖核蛋白类%成骨细胞
骨摺,不愈閤%骨形態髮生蛋白質類%覈糖覈蛋白類%成骨細胞
골절,불유합%골형태발생단백질류%핵당핵단백류%성골세포
Fractures,ununited%bone morphogenetic proteins%Ribonucleoproteins%Osteoblasts
目的 检测骨不连骨断端骨形态发生蛋白(BMP)、核心蛋白多糖成分的表达,观察单独应用和联合应用两种因子治疗骨不连的效果.方法 制作兔桡骨肥大型骨不连动物模型,通过大体观察、影像学和组织学检测,比较骨断端组织成分的变化.采用免疫组织化学染色、Real-time PCR、Western-Blot检测骨不连接区组织中BMP、核心蛋白多糖的表达.分别用单纯BMP(A组)、纤维蛋白胶复合BMP和核心蛋白多糖(B组)、单纯核心蛋白多糖(C组)和空白组溶解液(D组)局部注射骨不连接区,通过三维CT观察骨不连接区的成骨情况.结果 组织学检测示骨折愈合侧组织内大量新生骨组织和新生血管形成,骨不连侧组织内主要为纤维瘢痕组织.免疫组织化学检测显示骨折愈合侧可见核心蛋白多糖染色和BMP染色阳性;骨不连接侧无明显核心蛋白多糖表达,BMP-2表达阴性.骨愈合侧和骨不连侧核心蛋白多糖的相对表达量分别为2.402±0.260、1.313±0.187,差异有统计学意义(t=10.193.P=0.000).Westem-Blot检测示骨不连接侧组织BMP-2的表达较骨折愈合侧明显下降(t=69.007,P=0.000).A、B、C、D组局部注射治疗骨不连的成骨量CT值分别为551.40±41.85、674.40±36.55、471.40±27.91、309.20±147.86,差异有统计学意义(F=18.209,P=0.000).结论 骨不连形成后,由于BMP和核心蛋白多糖成分的减少,局部组织的抗纤维化能力和成骨能力下降,联合应用外源性BMP和核心蛋白多糖能促进骨不连接区陈旧性组织成骨.
目的 檢測骨不連骨斷耑骨形態髮生蛋白(BMP)、覈心蛋白多糖成分的錶達,觀察單獨應用和聯閤應用兩種因子治療骨不連的效果.方法 製作兔橈骨肥大型骨不連動物模型,通過大體觀察、影像學和組織學檢測,比較骨斷耑組織成分的變化.採用免疫組織化學染色、Real-time PCR、Western-Blot檢測骨不連接區組織中BMP、覈心蛋白多糖的錶達.分彆用單純BMP(A組)、纖維蛋白膠複閤BMP和覈心蛋白多糖(B組)、單純覈心蛋白多糖(C組)和空白組溶解液(D組)跼部註射骨不連接區,通過三維CT觀察骨不連接區的成骨情況.結果 組織學檢測示骨摺愈閤側組織內大量新生骨組織和新生血管形成,骨不連側組織內主要為纖維瘢痕組織.免疫組織化學檢測顯示骨摺愈閤側可見覈心蛋白多糖染色和BMP染色暘性;骨不連接側無明顯覈心蛋白多糖錶達,BMP-2錶達陰性.骨愈閤側和骨不連側覈心蛋白多糖的相對錶達量分彆為2.402±0.260、1.313±0.187,差異有統計學意義(t=10.193.P=0.000).Westem-Blot檢測示骨不連接側組織BMP-2的錶達較骨摺愈閤側明顯下降(t=69.007,P=0.000).A、B、C、D組跼部註射治療骨不連的成骨量CT值分彆為551.40±41.85、674.40±36.55、471.40±27.91、309.20±147.86,差異有統計學意義(F=18.209,P=0.000).結論 骨不連形成後,由于BMP和覈心蛋白多糖成分的減少,跼部組織的抗纖維化能力和成骨能力下降,聯閤應用外源性BMP和覈心蛋白多糖能促進骨不連接區陳舊性組織成骨.
목적 검측골불련골단단골형태발생단백(BMP)、핵심단백다당성분적표체,관찰단독응용화연합응용량충인자치료골불련적효과.방법 제작토뇨골비대형골불련동물모형,통과대체관찰、영상학화조직학검측,비교골단단조직성분적변화.채용면역조직화학염색、Real-time PCR、Western-Blot검측골불련접구조직중BMP、핵심단백다당적표체.분별용단순BMP(A조)、섬유단백효복합BMP화핵심단백다당(B조)、단순핵심단백다당(C조)화공백조용해액(D조)국부주사골불련접구,통과삼유CT관찰골불련접구적성골정황.결과 조직학검측시골절유합측조직내대량신생골조직화신생혈관형성,골불련측조직내주요위섬유반흔조직.면역조직화학검측현시골절유합측가견핵심단백다당염색화BMP염색양성;골불련접측무명현핵심단백다당표체,BMP-2표체음성.골유합측화골불련측핵심단백다당적상대표체량분별위2.402±0.260、1.313±0.187,차이유통계학의의(t=10.193.P=0.000).Westem-Blot검측시골불련접측조직BMP-2적표체교골절유합측명현하강(t=69.007,P=0.000).A、B、C、D조국부주사치료골불련적성골량CT치분별위551.40±41.85、674.40±36.55、471.40±27.91、309.20±147.86,차이유통계학의의(F=18.209,P=0.000).결론 골불련형성후,유우BMP화핵심단백다당성분적감소,국부조직적항섬유화능력화성골능력하강,연합응용외원성BMP화핵심단백다당능촉진골불련접구진구성조직성골.
Objective To investigate expressions of BMP and decorin at the broken ends of bone nonunion after fracture and effects of percutaneous injection of exogenous BMP and decorin, alone or in combination, on treatment of nonunion.Methods Radial nonunion models were made in rabbits.Changes in tissue components at broken ends of the fractured bone were observed through microscopy, radiology and histology.The expressions of BMP and decorin were detected by immunohistochemistry, real-time PCR and Westem-Blot.Fibrin sealant(FS)was adopted for percutaneous injections of exogenous BMP and decorin, alone or in combination, into the bone nonunion sites.Ossification in the area of nonunion was observed by 3D CT 4 weeks after the injection.Results In areas of bone nonunion, the active cells that could differentiate into osteoblasts obviously diminished and the expressions of BMP and decorin, compared with those in the normal bone, decreased obviously(P<0.05).Injection of both BMP and decorin were more effective than injection of either of them alone in treatment of nonunion.Conclusions Because of an obvious decrease of BMP and decorin, local tissues in bone nonunion tend to show less anti-fibrosis and ossification abilities.Therefore, a combined injection of exogenous BMP and decorin can promote healing of the bone nonunion.
