中华消化杂志
中華消化雜誌
중화소화잡지
Chinese Journal of Digestion
2008年
6期
385-387
,共3页
宋正己%王吉耀%涂传涛%王逸清%方国汀
宋正己%王吉耀%塗傳濤%王逸清%方國汀
송정기%왕길요%도전도%왕일청%방국정
肝硬化%整合素avβ3%肝星状细胞
肝硬化%整閤素avβ3%肝星狀細胞
간경화%정합소avβ3%간성상세포
Liver cirrhosis%Integrin avβ3%Hepatic stellate cell
目的 研究整合素avβ3在肝星状细胞和纤维化肝组织中的表达,探讨能反映肝星状细胞活化状态的表型受体.方法 分离大鼠肝星状细胞,体外培养激活.硫代乙酰胺175 mg/kg每周2次,连续12周腹腔注射诱导大鼠肝纤维化.免疫染色观察细胞和组织中整合avβ3和a-平滑肌肌动蛋白(a-SMA)的表达.实时PCR和Western印迹法分析avβ3在星状细胞中、正常和肝纤维化组织中的表达.结果 活化星状细胞中avβ3表达明显上调,与第1天相比,第14天的mRNA和蛋白质水平分别增加18倍和5.2倍.免疫染色整合素avβ3成簇极性表达于活化星状细胞的胞膜.肝纤维化模型中avβ3在mRNA和蛋白水平明显高于正常对照(t=2.39,P＜0.05;t=2.74,P＜0.05),正常肝组织中a-SMA和avβ3局限表达于门静脉周围,肝纤维化组织中a-SMA和avβ3表达于扩大的门管区和纤维间隔.结论 体内外活化星状细胞中整合素avβ3表达水平上调,它是星状细胞活化的表型受体,参与肝纤维化的发生.
目的 研究整閤素avβ3在肝星狀細胞和纖維化肝組織中的錶達,探討能反映肝星狀細胞活化狀態的錶型受體.方法 分離大鼠肝星狀細胞,體外培養激活.硫代乙酰胺175 mg/kg每週2次,連續12週腹腔註射誘導大鼠肝纖維化.免疫染色觀察細胞和組織中整閤avβ3和a-平滑肌肌動蛋白(a-SMA)的錶達.實時PCR和Western印跡法分析avβ3在星狀細胞中、正常和肝纖維化組織中的錶達.結果 活化星狀細胞中avβ3錶達明顯上調,與第1天相比,第14天的mRNA和蛋白質水平分彆增加18倍和5.2倍.免疫染色整閤素avβ3成簇極性錶達于活化星狀細胞的胞膜.肝纖維化模型中avβ3在mRNA和蛋白水平明顯高于正常對照(t=2.39,P＜0.05;t=2.74,P＜0.05),正常肝組織中a-SMA和avβ3跼限錶達于門靜脈週圍,肝纖維化組織中a-SMA和avβ3錶達于擴大的門管區和纖維間隔.結論 體內外活化星狀細胞中整閤素avβ3錶達水平上調,它是星狀細胞活化的錶型受體,參與肝纖維化的髮生.
목적 연구정합소avβ3재간성상세포화섬유화간조직중적표체,탐토능반영간성상세포활화상태적표형수체.방법 분리대서간성상세포,체외배양격활.류대을선알175 mg/kg매주2차,련속12주복강주사유도대서간섬유화.면역염색관찰세포화조직중정합avβ3화a-평활기기동단백(a-SMA)적표체.실시PCR화Western인적법분석avβ3재성상세포중、정상화간섬유화조직중적표체.결과 활화성상세포중avβ3표체명현상조,여제1천상비,제14천적mRNA화단백질수평분별증가18배화5.2배.면역염색정합소avβ3성족겁성표체우활화성상세포적포막.간섬유화모형중avβ3재mRNA화단백수평명현고우정상대조(t=2.39,P＜0.05;t=2.74,P＜0.05),정상간조직중a-SMA화avβ3국한표체우문정맥주위,간섬유화조직중a-SMA화avβ3표체우확대적문관구화섬유간격.결론 체내외활화성상세포중정합소avβ3표체수평상조,타시성상세포활화적표형수체,삼여간섬유화적발생.
Objective To investigate the expression of integrin avβ3 in both of hepatic stellate cell (HSC)and fibrotic liver tissue,and to demonstrate whether integrin avβ3 is a phenotypical receptor of activated HSC.Methods HSC were isolated from Sprague-Dawley rats and activated by prolonged cultural.The rats were injected with 175 mg of thioacetamide twice a week for 12 weeks to induce liver fibosis.The expressions of avβ3 and a-SMA were identified by immunocytochemical staining.The expression of avβ3 in HSC,normal and fibrotic tissues were examined by real-time PCR and Western blot.Results The expression of avβ3 in activated HSC were up regulated and levels of mRNA and protein were increased to 18 and 5.2 folds on day 14 compared with day 1 and were also higher than control(t=2.39,P＜0.05;t=2.74,P＜0.05).The immunochemistry staining showed that integrin avβ3 was expressed on membrane of activated HSCs.The avβ3 and a-SMA were expressed in portal vein in normal liver,but were also expressed in portal and fibrotic.Conclusions The integrin avβ3 is up-regulated in activated HSC both in vitro and in vivo.It is a phenotypical receptor of activated HSC which involved in liver fibrosis.
