CAJ | 학술논문

目的探讨丹参注射液对缺氧培养大鼠神经干细胞的凋亡及Caspase-3活性的影响,以进一步明确丹参注射液神经保护作用的分子机制.方法体外培养新生大鼠海马神经干细胞,将其分为正常对照组,缺氧培养组及丹参注射液处理组.Hoechst染色后荧光显微镜下观察并计算细胞凋亡率:比色法检测各组细胞Caspase-3的相对活性.结果缺氧培养大鼠神经干细胞的细胞凋亡率(30.12%±2.09%)及Caspase-3活性(3.85±0.41)均较正常对照组(2.75%±0.28%,1.16±0.07)明显升高,差异有统计学意义(P＜0.05);施加丹参注射液后,大鼠神经干细胞的细胞凋亡率(9.16%±1.34%)和Caspase-3活性(1.50±0.09)均较缺氧培养组明显下降,差异有统计学意义(P＜0.05).结论丹参注射液可对抗缺氧损伤所致的神经干细胞凋亡,从而起到神经保护作用.
목적 탐토단삼주사액대결양배양대서신경간세포적조망급Caspase-3활성적영향,이진일보명학단삼주사액신경보호작용적분자궤제.방법 체외배양신생대서해마신경간세포,장기분위정상대조조,결양배양조급단삼주사액처리조.Hoechst염색후형광현미경하관찰병계산세포조망솔:비색법검측각조세포Caspase-3적상대활성.결과 결양배양대서신경간세포적세포조망솔(30.12%±2.09%)급Caspase-3활성(3.85±0.41)균교정상대조조(2.75%±0.28%,1.16±0.07)명현승고,차이유통계학의의(P＜0.05);시가단삼주사액후,대서신경간세포적세포조망솔(9.16%±1.34%)화Caspase-3활성(1.50±0.09)균교결양배양조명현하강,차이유통계학의의(P＜0.05).결론단삼주사액가대항결양손상소치적신경간세포조망,종이기도신경보호작용.
Objective To explore the effects of salvia miltiorrhizae (SM) injection on the apoptosis of cultured rat neuronal stem cells induced by hypoxia and the activity of Caspase-3, in order to provide the further evidence for the molecular mechanism of neuroprotection of SM injection. Methods The neuronal stem cells from neonatal rat hippocampus were cultured and divided randomly into normal control group, hypoxia group and SM treatment group. After Hoechst staining, the apoptotic morphological change and apoptosis percentage were observed under fluorescence microscope. The activities of Caspase-3 in the 3 groups were evaluated by the colorimetric assay. Results Compared with normal control group [(2.75±0.28)%, 1.16±0.07], the percentage of apoptosis and the activity of Caspase-3 were increased significantly in neuronal stem cells cultured in hypoxia [(30.12%±2.09)%,3.85±0.41, P<0.05). Application of SM injection reduced markedly the percentage of apoptosis and the activity of Caspase-3 of the neuronal stem cells cultured in hypoxia [(9.16±1.34)%, 1.50±0.09, P<0.05].Conclusion SM injection can depress the apoptosis of the rat neuronal stem cells induced by hypoxia,so as to exert the neuroprotection.