CAJ | 학술논문

目的证实γδ T细胞在呼吸道合胞病毒(respiratory syncytial virus,RSV)不同感染时间所致哮喘鼠不同气道炎症反应中的作用.方法建立致敏前后不同时间感染RSV的哮喘动物模型,HE染色观察哮喘鼠肺炎症反应；real-time RT-PCR法检测γδ T细胞Th1/Th2型细胞因子IFN-γ和IL-4 mRNA表达；流式细胞仪分析脾及肺组织中γδ T细胞数量；γδ T细胞过继回输进一步明确其功能.结果致敏前感染RSV显著减轻哮喘鼠肺炎症；减少脾及肺组织细胞中γδ T细胞总数及其活化细胞数量；增加γδ T细胞IFN-γ mRNA表达；降低IL-4 mRNA表达,但致敏后感染RSV对哮喘鼠肺炎症反应及ΥδT细胞数量和生物学活性无明显影响.尾静脉回输γδ T细胞导致模型鼠肺炎症反应明显增强,提示γδ T细胞参与哮喘鼠气道炎症的发生发展.结论致敏前RSV感染可能通过影响γδ T细胞数量及其生物学活性改变变应原诱发的气道炎症反应.
목적 증실γδ T세포재호흡도합포병독(respiratory syncytial virus,RSV)불동감염시간소치효천서불동기도염증반응중적작용.방법 건립치민전후불동시간감염RSV적효천동물모형,HE염색관찰효천서폐염증반응；real-time RT-PCR법검측γδ T세포Th1/Th2형세포인자IFN-γ화IL-4 mRNA표체；류식세포의분석비급폐조직중γδ T세포수량；γδ T세포과계회수진일보명학기공능.결과 치민전감염RSV현저감경효천서폐염증；감소비급폐조직세포중γδ T세포총수급기활화세포수량；증가γδ T세포IFN-γ mRNA표체；강저IL-4 mRNA표체,단치민후감염RSV대효천서폐염증반응급ΥδT세포수량화생물학활성무명현영향.미정맥회수γδ T세포도치모형서폐염증반응명현증강,제시γδ T세포삼여효천서기도염증적발생발전.결론 치민전RSV감염가능통과영향γδ T세포수량급기생물학활성개변변응원유발적기도염증반응.
Objective To investigate whether γδ T cells act as a regulatory factor during respiratory syncytial virus (RSV) infections was responsible for the subsequent changes in asthmatic-type inflammation in allergic mice.Methods Mice were sensitized and challenged with OVA,and infected intranasaly with RSV before or after OVA sensitization.Lung sections were stained with HE for determination of inflammatory reaction.Real-time RT-PCR was used to analyze the expression of cytokine mRNA of γδ T cells in the lung and spleen of tested mice.The number of γδ T cells in the spleen and lung of BALB/c mice was determined by flow cytometry.Adoptive transfer of γδ T cells was performed to identify the role of γδ T cells in allergic asthma.Results OVA-sensitized and challenged mice exhibited significantly peribronchial inflammation with larger number of mononuclear cells and granulocytes in the lung tissue sections.RSV infection before OVA-sensitization diminished the grade of inflammatory responses induced by OVA treatment.The expression of IFN-γ mRNA was increased siguificantly in RSV-infected,OVA-sensitized mice.In contrast,the level of IL-4 mRNA was diminished.The number of total γδ T cells as well as activated γδ T cells was decreased in the spleen and lung of OVA-sensitized mice by prior RSV infection.Adoptive transfer of γδ T cells obtained from OVA-sensitized and challenged mice induced a slight inflammation in the lung of normal mice,and enhanced inflammatory responses in RSV-infected OVA-sensitized mice.Conclusion γδ T cells may play an important role in the development of allergen-induced allergic airway inflammation.