CAJ | 학술논문

目的研究钙离子拮抗剂维拉帕米及尼卡地平对体外培养的瘢痕组织的成纤维细胞增殖的抑制作用,同时对两药药效进行比较,观测两药的作用特点. 方法四甲基偶氮唑(MTT)比色法检测两药对瘢痕组织的成纤维细胞增殖的影响:向对数生长期的患者病理性瘢痕组织的成纤维细胞中分别加入不同浓度维拉帕米及尼卡地平(150、100、50、10、0μmol/L),分别在用药后24、72、120 h进行MTT法检测,将吸光度值(A值)换算为生长抑制率,根据直线回归法计算出各时间点的半数有效抑制浓度(IC50)并进行统计学比较.结果维拉帕米及尼卡地平均显示很强的对瘢痕组织成纤维细胞增殖的抑制作用.但作用特点各有不同,维拉帕米在早期作用强于尼卡地平(P＜0.05).而随时间延长,在3～5 d时反而减弱,而尼卡地平则显示出较好的时间依赖性,在5 d时其抑制作用明显强于维拉帕米(P＜0.05).结论维拉帕米及尼卡地平对瘢痕组织成纤维细胞增殖抑制作用的时效不同,为两药联合应用治疗瘢痕提供了理论依据.
목적 연구개리자길항제유랍파미급니잡지평대체외배양적반흔조직적성섬유세포증식적억제작용,동시대량약약효진행비교,관측량약적작용특점. 방법 사갑기우담서(MTT)비색법검측량약대반흔조직적성섬유세포증식적영향:향대수생장기적환자병이성반흔조직적성섬유세포중분별가입불동농도유랍파미급니잡지평(150、100、50、10、0μmol/L),분별재용약후24、72、120 h진행MTT법검측,장흡광도치(A치)환산위생장억제솔,근거직선회귀법계산출각시간점적반수유효억제농도(IC50)병진행통계학비교.결과유랍파미급니잡지평균현시흔강적대반흔조직성섬유세포증식적억제작용.단작용특점각유불동,유랍파미재조기작용강우니잡지평(P＜0.05).이수시간연장,재3～5 d시반이감약,이니잡지평칙현시출교호적시간의뢰성,재5 d시기억제작용명현강우유랍파미(P＜0.05).결론유랍파미급니잡지평대반흔조직성섬유세포증식억제작용적시효불동,위량약연합응용치료반흔제공료이론의거.
Objective To study the inhibitory effect of verapamil and nicardipine on human scar fibroblast in serum-free culture and to compare the effectness of the two drugs.Methods We used MTT method to detect the effect of two drugs on human scar fibroblast proliferation:adding verapamil and nicardipine with different concentrations in the culture of fibroblasts which were in logarithmic growth phase (150,100,50,10,0μmol/L).After 24,72,and 120 h,we used MTT method to detect the cell proliferation,and converted the absorbance into growth inhibitory ratio.Results Verapamil and nicardipine showed the definite inhibition on the hypertrophic scar fibroblast (HSFB) and keloid fibroblast (KDFB) which were cultured in vitro.There was some difference in the action feature.In the earlier period,the effect of verapamil was powerful than that of nicardipine.With time,the effect did not reinforce.When fibroblast had been cultured for three to five days,the inhibition became weak.But nicardipine showed lasting inhibition on fibroblast proliferation.Conclusion Combination of verapamil with nicardipine may be a valuable method in the treatment of scar.