中华预防医学杂志
中華預防醫學雜誌
중화예방의학잡지
CHINESE JOURNAL OF
2009年
2期
137-140
,共4页
闫笑梅%顾一心%何利华%张慧芳%张建中
閆笑梅%顧一心%何利華%張慧芳%張建中
염소매%고일심%하리화%장혜방%장건중
细菌分型技术%甲氧西林抗药性%葡萄球菌%金黄色
細菌分型技術%甲氧西林抗藥性%葡萄毬菌%金黃色
세균분형기술%갑양서림항약성%포도구균%금황색
Bacterial typing techniques%Methicillin resistance%Staphylococcus aureus
目的 对2000年和2005年耐甲氧西林金黄色葡萄球菌(methicillin-resistant Staphylococcus aureus,MRSA)进行多位点序列分析(multilocus sequencing typing,MLST),初步了解MRSA的分子分型特征.方法 采用基因测序的方法 分别对29株MRSA和2株甲氧西林敏感金黄色葡萄球菌(methicillin-sensitive Staphylococcus aureus,MSSA)的7个等位基因进行序列分析,同时进行网上数据库比对,得到每个菌株相应的等位基因号.结果 2000年选取的12株MRSA均为序列型(sequence type,ST)239,2005年的17株MRSA中10株仍为ST239(58.82%,10/17),同时也出现了新的型别ST5(41.18%,7/17).在选取菌株范围内ST239为主要多位点序列分析型别(75.86%,22/29),其次为ST5(24.14%,7/29).2株MSSA分型结果分别为ST6和ST630.结论 ST239和ST5为本次研究的优势型别,MRSA与MSSA在多位点序列分析上存在极大的差异.
目的 對2000年和2005年耐甲氧西林金黃色葡萄毬菌(methicillin-resistant Staphylococcus aureus,MRSA)進行多位點序列分析(multilocus sequencing typing,MLST),初步瞭解MRSA的分子分型特徵.方法 採用基因測序的方法 分彆對29株MRSA和2株甲氧西林敏感金黃色葡萄毬菌(methicillin-sensitive Staphylococcus aureus,MSSA)的7箇等位基因進行序列分析,同時進行網上數據庫比對,得到每箇菌株相應的等位基因號.結果 2000年選取的12株MRSA均為序列型(sequence type,ST)239,2005年的17株MRSA中10株仍為ST239(58.82%,10/17),同時也齣現瞭新的型彆ST5(41.18%,7/17).在選取菌株範圍內ST239為主要多位點序列分析型彆(75.86%,22/29),其次為ST5(24.14%,7/29).2株MSSA分型結果分彆為ST6和ST630.結論 ST239和ST5為本次研究的優勢型彆,MRSA與MSSA在多位點序列分析上存在極大的差異.
목적 대2000년화2005년내갑양서림금황색포도구균(methicillin-resistant Staphylococcus aureus,MRSA)진행다위점서렬분석(multilocus sequencing typing,MLST),초보료해MRSA적분자분형특정.방법 채용기인측서적방법 분별대29주MRSA화2주갑양서림민감금황색포도구균(methicillin-sensitive Staphylococcus aureus,MSSA)적7개등위기인진행서렬분석,동시진행망상수거고비대,득도매개균주상응적등위기인호.결과 2000년선취적12주MRSA균위서렬형(sequence type,ST)239,2005년적17주MRSA중10주잉위ST239(58.82%,10/17),동시야출현료신적형별ST5(41.18%,7/17).재선취균주범위내ST239위주요다위점서렬분석형별(75.86%,22/29),기차위ST5(24.14%,7/29).2주MSSA분형결과분별위ST6화ST630.결론 ST239화ST5위본차연구적우세형별,MRSA여MSSA재다위점서렬분석상존재겁대적차이.
Objective To analyze multilocus sequence typing (MIST) of methicillin-resistant Staphylococcus aurens (MRSA) strains in 2000 and 2005, and get a primary knowledge of MIST Characterization of MRSA. Methods Sequence analysis was conducted on seven allelic genes of 29 methicillin-resistant Staphylococcus aureus strains and 2 methicillin-sensitive Staphylococcus aureus (MSSA) strains and the allelic profiles were gained from internet database. Results All 12 MRSA strains in 2000 were sequence type(ST) 239 and 10 MRSA strains in 2005 were ST239,while 7 MRSA strains in 2005 were new types,ST5 (41.18% ,7/17). ST6 and ST630 were allelic profiles of 2 MSSA strains. ST239 was the most prevalent allelie profile(75. 86% ,22/29), while ST5 was the second prevalent allelic profile(24. 14% ,7/ 29) among all isolates. Conclusion ST239 and ST5 are the most prevalent MRSA clones in this research. MRSA strains have different allelic profile from MSSA strains. MIST might provide an unambiguous method for assigning MRSA and MSSA isolates to known clones or assigning them as novel clones via the internet. Further studies need to be taken by increasing strains.
