CAJ | 학술논문

研究了用A蛋白酶联免疫吸附检测法(PAs-ELsA)检测葡萄卷叶病毒的程序，提出了A蛋白、酶标A蛋白、抗体的适宜工作浓度及样品粗提液稀释倍数。通过对感病植株的幼叶、幼茎、老叶、老茎的检测研究，规范了PAS-ELIsA检测技术。应用该技术，对国家果树种质资源圃郑州葡萄圃的39份种质及郑州地区栽培的品种进行了检测，保存品种的带毒率76．9％，抗逆性较强的东亚种群带毒率55．6％，郑州地区栽培品种葡萄带毒率为45．0％。
연구료용A단백매련면역흡부검측법(PAs-ELsA)검측포도권협병독적정서，제출료A단백、매표A단백、항체적괄의공작농도급양품조제액희석배수。통과대감병식주적유협、유경、로협、로경적검측연구，규범료PAS-ELIsA검측기술。응용해기술，대국가과수충질자원포정주포도포적39빈충질급정주지구재배적품충진행료검측，보존품충적대독솔76．9％，항역성교강적동아충군대독솔55．6％，정주지구재배품충포도대독솔위45．0％。
The suitable concentration of protein- A, enzyme- labelled protein - A and antibody, and the dilution multipleof sample crude extract were ascertained in detecting grapevine leaf-- roll virus with young leaves and old leaves and youngstems and old stems by PAS- ELISA. Detection of 39 germ plasms in the Zhengzhou Grapevine Garden of the ChinesePomology Germplasm Gardens andcultivated grapevines in Zhengzhou Area by PAS- ELISA showed that the virused ratewas 76.9 % for the varieties preserved in Zhengzhou Grapevine Graden, 55.6 ％ for the Eastemn Asian species, and 45.0 % for the varieties cultivated in Zhengzhou Area.