CAJ | 학술논문

凝集素样氧化型低密度脂蛋白受体-1(LOX-1)是氧化型低密度脂蛋白(ox-LDL)的主要受体,它与动脉粥样硬化过程密切相关.通过RT-PCR从THP-1细胞系获得LOX-1 cDNA并被克隆到载体pPIC9K的α信号肽的下游.线性化的重组质粒转化Pichia pastoris GS115.阳性转化子经过BC液体培养基培养,上清液经聚丙烯酰胺凝胶电泳(SDS-PAGE)分析,出现1条在受体菌对照中不曾有的大小约43 kDa的蛋白带.重组蛋白的功能研究在进行中.
응집소양양화형저밀도지단백수체-1(LOX-1)시양화형저밀도지단백(ox-LDL)적주요수체,타여동맥죽양경화과정밀절상관.통과RT-PCR종THP-1세포계획득LOX-1 cDNA병피극륭도재체pPIC9K적α신호태적하유.선성화적중조질립전화Pichia pastoris GS115.양성전화자경과BC액체배양기배양,상청액경취병희선알응효전영(SDS-PAGE)분석,출현1조재수체균대조중불증유적대소약43 kDa적단백대.중조단백적공능연구재진행중.
Lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) is a major endothelial receptor for oxidized low-density lipoprotein (oxLDL), and is assumed to play an important role in atherosclerosis.LOX-1 cDNA was obtained from human THP-1 cells by RT-PCR and inserted into the downstream of α -signal peptide in pPIC9K. The linear form of the recombinant plasmid was transformed into Pichia pastoris GS115. The transformants were cultivated in BMMC liquid medium. SDS-PAGE analysis showed that a protein band with over Mr 43 kDa was in the transformants but not in the host strain. Further function analysis of the protein is in progress.