CAJ | 학술논문

目的利用图像灰度的统计特征参数量化微重力条件下大鼠心肌细胞微管图像的形态变化,并研究在不同条件下这种变化的特点.方法对模拟微重力条件下培养的乳鼠心肌细胞(回转组)和正常条件下培养的心肌细胞(对照组)细胞骨架的微管图像提取灰度统计特征,利用灰度方差、偏度及峰度等参数量化细胞骨架的灰度特征.结果在对24幅图像进行特征分析后,发现所选的参数对量化微管的灰度特征有不同程度的统计意义.利用灰度方差、偏度和峰度进行多元判决,发现这些参数可以很好地区分模拟微重力条件下培养的心肌细胞图像和正常条件下培养的心肌细胞图像,总的错误判决率达到16.7%.结论在微重力条件下心肌细胞骨架微管的形态变得弥散,利用灰度特征参数方差、偏度和峰度可以描述这种变化.
목적이용도상회도적통계특정삼수양화미중력조건하대서심기세포미관도상적형태변화,병연구재불동조건하저충변화적특점.방법 대모의미중력조건하배양적유서심기세포(회전조)화정상조건하배양적심기세포(대조조)세포골가적미관도상제취회도통계특정,이용회도방차、편도급봉도등삼수양화세포골가적회도특정.결과 재대24폭도상진행특정분석후,발현소선적삼수대양화미관적회도특정유불동정도적통계의의.이용회도방차、편도화봉도진행다원판결,발현저사삼수가이흔호지구분모의미중력조건하배양적심기세포도상화정상조건하배양적심기세포도상,총적착오판결솔체도16.7%.결론 재미중력조건하심기세포골가미관적형태변득미산,이용회도특정삼수방차、편도화봉도가이묘술저충변화.
Objective To quantify the images of the microtubules in fetal rat cardiac myocytes under simulated microgravity by utilizing the characteristic parameters of image gray, and to study their morphological change. Method Gray characteristic of the microtubules in fetal rat cardiac myocytes was quantified in both simulated mircogravity and control conditions by variance, skewness, and kurtosis. Result From feature analysis of 24 images, the characteristic parameters selected here were proved to be effective. Good result was obtained when discrimination between simulated microgravity group and control group was made by multivariate analysis with these parameters. The total false verdict rate even reached 16.7% when using multivariate analysis with these parameters. Conclusion The morphology of the microtubules in cardiac myocytes cytoskeleton became diffused under simulated microgravity, and the quantitative analysis of gray parameters (variance, skewness, kurtosis) described the variation satisfactorily.