CAJ | 학술논문

从农药厂污水处理池中分离得到一株氯氰菊酯降解菌,在30℃,pH 7.0的条件下,无机盐培养基中100mg/L的氯氰菊酯,经过7.5天,能够降解大约52.3%,外加碳源能够明显提高其降解性能.生理生化实验结合16S rDNA,gyrB和GyrB的系统发育分析,将其归为Gordonia菌属.在16S rDNA水平上,其与G.amicalis DSM44461和G.hydrophobica DSM44015<'T>的相似值最高,为98.1%;而在gyrB和GyrB水平上,其与G.hydrophobica JCM10086的相似值最高,分别为86.8%和91.1%.通过对所构建的系统发育树进行评估,表明16S rDNA序列适用于将分离菌株鉴定到属的水平上,而gyrB和GyrB更适用于在属内种的水平上进行系统发育的分析.
종농약엄오수처리지중분리득도일주록청국지강해균,재30℃,pH 7.0적조건하,무궤염배양기중100mg/L적록청국지,경과7.5천,능구강해대약52.3%,외가탄원능구명현제고기강해성능.생리생화실험결합16S rDNA,gyrB화GyrB적계통발육분석,장기귀위Gordonia균속.재16S rDNA수평상,기여G.amicalis DSM44461화G.hydrophobica DSM44015<'T>적상사치최고,위98.1%;이재gyrB화GyrB수평상,기여G.hydrophobica JCM10086적상사치최고,분별위86.8%화91.1%.통과대소구건적계통발육수진행평고,표명16S rDNA서렬괄용우장분리균주감정도속적수평상,이gyrB화GyrB경괄용우재속내충적수평상진행계통발육적분석.
A cypermethrin - degrading bacterium named as Gordonia sp. D2 was isolated from activated sludge of a wastewater treatment biore-actor. At 30 ℃ and pH 7.0, the removal rate of 100 mg cypennethrin L-1 was approximately 52.3% in mineral salts medium within 7.5 days, and extra carbon source addition could enhance the biodegradation. The isolate was identified as Gordonia genus through physio - bio- chemical identification combined with the phylogenetic analyses based on 16S rRNA, gyrB nucleotide and GyrB amino acid sequences. 16S rRNA sequences of strain D2 showed the highest similarity to G. amicalis DSM44461 and G. hydrophobica DSM44015T, with the same value of 98.1% ,meanwhile, D2 shows the highest similarity to G. hydrophobica JCA10086 with the value of 86.8% and 91.1% based on gyrB and GyrB. Three phylogenetic dendrograms were made, and results showed that 16S rRNA gene is suit for identifying the isolate to the genus level, gyrB gene and GyrB amino acid sequences are more adapted for phylogenetic analysis.